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Rheb antibody [GT39810]

Anti-Rheb antibody [GT39810] used in IHC (Paraffin sections) (IHC-P). GTX634335
Anti-Rheb antibody [GT39810] used in Western Blot (WB). GTX634335
Anti-Rheb antibody [GT39810] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634335
Anti-Rheb antibody [GT39810] used in IHC (Paraffin sections) (IHC-P). GTX634335

Cat. No. GTX634335

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT39810

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Rheb products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

SK-N-SH

Predict Reactivity

Zebrafish, Sheep, Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rheb. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888438

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Ras homolog, mTORC1 binding , RHEB2

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side

Background

This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Rheb antibody [GT39810] used in IHC (Paraffin sections) (IHC-P). GTX634335

GTX634335 IHC-P Image

Rheb antibody [GT39810] detects Rheb protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Rheb stained by Rheb antibody [GT39810] (GTX634335) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rheb antibody [GT39810] used in Western Blot (WB). GTX634335

GTX634335 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Rheb antibody [GT39810] (GTX634335) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Rheb antibody [GT39810] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634335

GTX634335 ICC/IF Image

Rheb antibody [GT39810] detects Rheb protein at cytoplasm by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Rheb stained by Rheb antibody [GT39810] (GTX634335) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-Rheb antibody [GT39810] used in IHC (Paraffin sections) (IHC-P). GTX634335

GTX634335 IHC-P Image

Rheb antibody [GT39810] detects Rheb protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Rheb stained by Rheb antibody [GT39810] (GTX634335) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations