APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
2 - 4 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Rat Heart Tissue Lysate(GTX28912)
Product Note
Post-translationally modified Rheb is sometimes observed at higher molecular weight.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Rabbit Rheb polyclonal antibody was raised against a 14 amino acid synthetic peptide from the amino terminus of human Rheb.The immunogen is located within the first 50 amino acids of Rheb.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Ras homolog, mTORC1 binding , RHEB2
Cellular Localization
Golgi apparatus membrane,Cytoplasm, cytosol,Endoplasmic reticulum membrane
Background
This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX31712 WB Image
WB analysis of rat heart tissue lysate using GTX31712 Rheb antibody. Working concentration : (A) 2 and (B) 4 μg/ml
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REFERENCE
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REVIEW
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