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Rheb antibody

Anti-Rheb antibody used in Western Blot (WB). GTX31712

Cat No. GTX31712

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 2 - 4 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

Rat Heart Tissue Lysate(GTX28912)

Specificity/Sensitivity

Post-translationally modified Rheb is sometimes observed at higher molecular weight.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Rabbit Rheb polyclonal antibody was raised against a 14 amino acid synthetic peptide from the amino terminus of human Rheb.The immunogen is located within the first 50 amino acids of Rheb.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Ras homolog, mTORC1 binding , RHEB2

Cellular Localization

Golgi apparatus membrane,Cytoplasm, cytosol,Endoplasmic reticulum membrane

Background

This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Rheb antibody used in Western Blot (WB). GTX31712

GTX31712 WB Image

WB analysis of rat heart tissue lysate using GTX31712 Rheb antibody.
Working concentration : (A) 2 and (B) 4 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339