Rho GDI alpha antibody [2G3]

Cat. No. GTX60618

Host	Mouse
Clonality	Monoclonal
Clone Name	2G3
Isotype	IgG1
Application	WB, ICC/IF, FACS, ELISA
Reactivity	Human, Mouse, Rat, Monkey
Package	100 μg ($399)

See all Rho GDI alpha products

Datasheet
Document

View product citations for antibody GTX60618 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human Rho GDI alpha (AA: FULL(1-204)) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rho GDP dissociation inhibitor alpha , GDIA1 , HEL-S-47e , NPHS8 , RHOGDI , RHOGDI-1

Cellular Localization

Cytoplasm

Background

This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Database

Gene ID: 396 ARHGDIA
UniProt: P52565 ARHGDIA

Research Area

DATA IMAGES

GTX60618 FACS Image

FACS analysis of HeLa cells using GTX60618 Rho GDI alpha antibody [2G3].
Green : Rho GDI alpha
Red : negative control

GTX60618 ELISA Image

ELISA analysis of antigen using GTX60618 Rho GDI alpha antibody [2G3].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

GTX60618 WB Image

WB analysis of Jurkat (1), HeLa (2), NIH3T3 (3), C6 (4), K562 (5), and COS7 (6) cell lysate using GTX60618 Rho GDI alpha antibody [2G3].

GTX60618 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX60618 Rho GDI alpha antibody [2G3].
Green : Rho GDI alpha
Blue: DRAQ5 fluorescent DNA dye

REFERENCE

There are currently no references for Rho GDI alpha antibody [2G3] (GTX60618). Be the first to share your publications with this product.

Submit a Reference

REVIEW

There are currently no reviews for Rho GDI alpha antibody [2G3] (GTX60618). Be the first to share your experience with this product.

Submit a Review

SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty