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Rho GDI alpha antibody [N1C3]

GTX101428_39721_IHC_19011506_232.jpg
GTX101428_39721_WB_M_brain_19011506_417.jpg
GTX101428_WB_19011506_288.jpg

Cat No. GTX101428

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

NT2D , IMR32 , Jurkat , HeLa , mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rho GDI alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Rho Gdp Dissociation Inhibitor Alpha,Gdia1,Hel-S-47E,Nphs8,Rhogdi,Rhogdi-1,Arhgdia

Cellular Localization

Cytoplasm

Background

Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (Leffers et al., 1993 [PubMed 8262133]).[supplied by OMIM]

Database

DATA IMAGES
GTX101428_39721_IHC_19011506_232.jpg

GTX101428 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HUH7 xenograft, using RhoGDI(GTX101428) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101428_39721_WB_M_brain_19011506_417.jpg

GTX101428 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX101428 diluted at 1:10000

GTX101428_WB_19011506_288.jpg

GTX101428 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
12% SDS PAGE
GTX101428 diluted at 1:500

REVIEW

Rho GDI alpha antibody [N1C3] Cat No. GTX101428

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 27-Jun-2018
Score :
Application Tested : WB
Sample : OEC-M1 cells
Amount used : 40 μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169