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Rho GDI alpha antibody

KO/KD
Anti-Rho GDI alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30064
Anti-Rho GDI alpha antibody used in Western Blot (WB). GTX30064

Cat. No. GTX30064

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all Rho GDI alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-204 of human ARHGDIA (NP_001172006.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rho GDP dissociation inhibitor alpha , GDIA1 , HEL-S-47e , NPHS8 , RHOGDI , RHOGDI-1

Cellular Localization

Cytoplasm

Background

This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Database

  • Gene ID: 396 ARHGDIA
  • UniProt: P52565 ARHGDIA

Research Area

DATA IMAGES

Anti-Rho GDI alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30064

GTX30064 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30064 Rho GDI alpha antibody.
Blue : DAPI

Anti-Rho GDI alpha antibody used in Western Blot (WB). GTX30064

GTX30064 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX30064 Rho GDI alpha antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399