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RhoA + RhoB + RhoC antibody [1A11-4G10]

Anti-RhoA + RhoB + RhoC antibody [1A11-4G10] used in IHC (Paraffin sections) (IHC-P). GTX31128
Anti-RhoA + RhoB + RhoC antibody [1A11-4G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31128
Anti-RhoA + RhoB + RhoC antibody [1A11-4G10] used in IHC (Paraffin sections) (IHC-P). GTX31128
Anti-RhoA + RhoB + RhoC antibody [1A11-4G10] used in IHC (Paraffin sections) (IHC-P). GTX31128
Anti-RhoA + RhoB + RhoC antibody [1A11-4G10] used in Immunoprecipitation (IP). GTX31128
Anti-RhoA + RhoB + RhoC antibody [1A11-4G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31128
Anti-RhoA + RhoB + RhoC antibody [1A11-4G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31128
Anti-RhoA + RhoB + RhoC antibody [1A11-4G10] used in Western Blot (WB). GTX31128

Cat No. GTX31128

Host

Mouse

Clonality

Monoclonal

Clone Name

1A11-4G10

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1500
ICC/IF 1:10-1:200
IHC-P 1:20-1:200
IP 2 μg
Not tested in other applications.

Specificity/Sensitivity

This antibody detects an ~20 kDa protein and shows reactivity to RhoA, RhoB, and RhoC.

PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 30% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length recombinant human Rho A

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-RhoA + RhoB + RhoC antibody [1A11-4G10] used in IHC (Paraffin sections) (IHC-P). GTX31128

GTX31128 IHC-P Image

IHC-P analysis of human hepatocarcinoma tissue using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-RhoA + RhoB + RhoC antibody [1A11-4G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31128

GTX31128 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

Anti-RhoA + RhoB + RhoC antibody [1A11-4G10] used in IHC (Paraffin sections) (IHC-P). GTX31128

GTX31128 IHC-P Image

IHC-P analysis of mouse breast tissue using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-RhoA + RhoB + RhoC antibody [1A11-4G10] used in IHC (Paraffin sections) (IHC-P). GTX31128

GTX31128 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-RhoA + RhoB + RhoC antibody [1A11-4G10] used in Immunoprecipitation (IP). GTX31128

GTX31128 IP Image

IP analysis of HeLa cell lysate using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
IP reaction : 2μg antibody / 750μg lysate
Loading : 25 μg
Dilution : 1:1000

Anti-RhoA + RhoB + RhoC antibody [1A11-4G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31128

GTX31128 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

Anti-RhoA + RhoB + RhoC antibody [1A11-4G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31128

GTX31128 ICC/IF Image

ICC/IF analysis of A431 cells using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

Anti-RhoA + RhoB + RhoC antibody [1A11-4G10] used in Western Blot (WB). GTX31128

GTX31128 WB Image

WB analysis of 25 μg of various whole cell lysates or 0.5 μg of purified protein (full-length His-RhoA, truncated GST-RhoB or full-length GST-RhoC fusion) per well using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
Dilution : 1:1000

REFERENCE

There are currently no references for RhoA + RhoB + RhoC antibody [1A11-4G10] (GTX31128). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for RhoA + RhoB + RhoC antibody [1A11-4G10] (GTX31128). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329