WB analysis of 25 μg of various whole cell lysates or 0.5 μg of purified protein (full-length His-RhoA, truncated GST-RhoB or full-length GST-RhoC fusion) per well using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
Dilution : 1:1000

IHC-P analysis of human hepatocarcinoma tissue using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

ICC/IF analysis of A431 cells using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

ICC/IF analysis of NIH-3T3 cells using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

IHC-P analysis of mouse breast tissue using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

ICC/IF analysis of NIH-3T3 cells using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

IP analysis of HeLa cell lysate using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
IP reaction : 2μg antibody / 750μg lysate
Loading : 25 μg
Dilution : 1:1000

IHC-P analysis of human prostate carcinoma tissue using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20