GeneTex
United States (US)

RhoA + RhoB + RhoC antibody [1A11-4G10]

GTX31128_1339_IHC-P_19011519_624.jpg
GTX31128_755_ICC-IF_19011519_883.jpg
GTX31128_1341_IHC-P_19011519_369.jpg
GTX31128_1340_IHC-P_19011519_930.jpg
GTX31128_1450_IP_19011519_431.jpg
GTX31128_757_ICC-IF_19011519_322.jpg
GTX31128_756_ICC-IF_19011519_937.jpg
GTX31128_1849_WB_19011519_288.jpg

Cat No. GTX31128

Host Mouse
Clonality Monoclonal
Clone Name 1A11-4G10
Isotype IgG1
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1500
ICC/IF 1:10-1:200
IHC-P 1:20-1:200
IP 2 μg
Not tested in other applications.

Specificity/Sensitivity

This antibody detects an ~20 kDa protein and shows reactivity to RhoA, RhoB, and RhoC.
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 30% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length recombinant human Rho A

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX31128_1339_IHC-P_19011519_624.jpg

GTX31128 IHC-P Image

IHC-P analysis of human hepatocarcinoma tissue using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

GTX31128_755_ICC-IF_19011519_883.jpg

GTX31128 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

GTX31128_1341_IHC-P_19011519_369.jpg

GTX31128 IHC-P Image

IHC-P analysis of mouse breast tissue using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

GTX31128_1340_IHC-P_19011519_930.jpg

GTX31128 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

GTX31128_1450_IP_19011519_431.jpg

GTX31128 IP Image

IP analysis of HeLa cell lysate using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
IP reaction : 2μg antibody / 750μg lysate
Loading : 25 µg
Dilution : 1:1000

GTX31128_757_ICC-IF_19011519_322.jpg

GTX31128 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

GTX31128_756_ICC-IF_19011519_937.jpg

GTX31128 ICC/IF Image

ICC/IF analysis of A431 cells using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

GTX31128_1849_WB_19011519_288.jpg

GTX31128 WB Image

WB analysis of 25 µg of various whole cell lysates or 0.5 µg of purified protein (full-length His-RhoA, truncated GST-RhoB or full-length GST-RhoC fusion) per well using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10].
Dilution : 1:1000

Package List Price ($)
$ 319