ICC/IF analysis of HeLa cells using GTX31144 RhoA + RhoC antibody [1B3-4A10].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

IHC-P analysis of human hepatocarcinoma tissue using GTX31144 RhoA + RhoC antibody [1B3-4A10].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

WB analysis of 25 μg of various whole cell lysates or 0.5 μg of purified protein (RhoA, RhoB, and a GST-RhoC fusion) per well using GTX31144 RhoA + RhoC antibody [1B3-4A10].
Dilution : 1:1000

IHC-P analysis of human prostate carcinoma tissue using GTX31144 RhoA + RhoC antibody [1B3-4A10].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

ICC/IF analysis of A431 cells using GTX31144 RhoA + RhoC antibody [1B3-4A10]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

IHC-P analysis of mouse liver tissue using GTX31144 RhoA + RhoC antibody [1B3-4A10].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

ICC/IF analysis of HeLa cells using GTX31144 RhoA + RhoC antibody [1B3-4A10]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

IP analysis of HeLa cell lysates using GTX31144 RhoA + RhoC antibody [1B3-4A10].
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1:1000