GeneTex
United States (US)

RhoA + RhoC antibody [1B3-4A10]

GTX31144_1344_IHC-P_19011519_678.jpg
GTX31144_1343_IHC-P_19011519_666.jpg
GTX31144_760_ICC-IF_19011519_892.jpg
GTX31144_1451_IP_19011519_329.jpg
GTX31144_759_ICC-IF_19011519_436.jpg
GTX31144_1850_WB_19011519_217.jpg
GTX31144_1342_IHC-P_19011519_309.jpg
GTX31144_758_ICC-IF_19011519_538.jpg

Cat No. GTX31144

Host Mouse
Clonality Monoclonal
Clone Name 1B3-4A10
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1500
ICC/IF 1:20-1:200
IHC-P 1:50-1:500
IP 2 μg
Not tested in other applications.

Specificity/Sensitivity

This antibody detects GTPases RhoA and RhoC, but does not react with RhoB.
PROPERTIES

Form

Liquid

Buffer

PBS, 30% glycerol, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length human recombinant RhoA protein.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX31144_1344_IHC-P_19011519_678.jpg

GTX31144 IHC-P Image

IHC-P analysis of human hepatocarcinoma tissue using GTX31144 RhoA + RhoC antibody [1B3-4A10].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

GTX31144_1343_IHC-P_19011519_666.jpg

GTX31144 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX31144 RhoA + RhoC antibody [1B3-4A10].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

GTX31144_760_ICC-IF_19011519_892.jpg

GTX31144 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31144 RhoA + RhoC antibody [1B3-4A10].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

GTX31144_1451_IP_19011519_329.jpg

GTX31144 IP Image

IP analysis of HeLa cell lysates using GTX31144 RhoA + RhoC antibody [1B3-4A10].
IP reaction : 2μg antibody / 500μg lysate
Dilution : 1:1000

GTX31144_759_ICC-IF_19011519_436.jpg

GTX31144 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31144 RhoA + RhoC antibody [1B3-4A10]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4°C

GTX31144_1850_WB_19011519_217.jpg

GTX31144 WB Image

WB analysis of 25 µg of various whole cell lysates or 0.5 µg of purified protein (RhoA, RhoB, and a GST-RhoC fusion) per well using GTX31144 RhoA + RhoC antibody [1B3-4A10].
Dilution : 1:1000

GTX31144_1342_IHC-P_19011519_309.jpg

GTX31144 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX31144 RhoA + RhoC antibody [1B3-4A10].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

GTX31144_758_ICC-IF_19011519_538.jpg

GTX31144 ICC/IF Image

ICC/IF analysis of A431 cells using GTX31144 RhoA + RhoC antibody [1B3-4A10]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4°C

Package List Price ($)
$ 319