GeneTex
United States (US)

RhoA antibody [1B8-1C7]

GTX31145_1853_WB_19011519_336.jpg
GTX31145_1852_WB_19011519_434.jpg
GTX31145_1851_WB_19011519_496.jpg

Cat No. GTX31145

Host Mouse
Clonality Monoclonal
Clone Name 1B8-1C7
Isotype IgG1
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Specificity/Sensitivity

This antibody detects recombinant RhoA, but not recombinant RhoB or recombinant RhoC.
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length recombinant His-tagged human RhoA

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ras Homolog Family Member A,Arh12,Arha,Rho12,Rhoh12,Rhoa

Cellular Localization

Cell cortex,Cell membrane,Cell projection,Cleavage furrow,Cytoplasm,Cytoskeleton,Lamellipodium,Midbody

Background

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES
GTX31145_1853_WB_19011519_336.jpg

GTX31145 WB Image

WB analysis of whole cell extracts (30 µg lysate) of Mouse Kidney (Lane 1), HeLa (Lane 2), NIH/3T3 (Lane 3), A-431 (Lane 4), PC-12 (Lane 5), Jurkat (Lane 6), and HEK-293 (Lane 7) using GTX31145 RhoA antibody [1B8-1C7].
Dilution : 1:500-1:2000

GTX31145_1852_WB_19011519_434.jpg

GTX31145 WB Image

WB analysis of human recombinant RhoA-GST (Lane 1), RhoB-GST (Lane 2) and RhoC-GST (Lane 3) using GTX31145 RhoA antibody [1B8-1C7].
Dilution : 2 µg/ml
Loading : 100 ng

GTX31145_1851_WB_19011519_496.jpg

GTX31145 WB Image

WB analysis of 40 ug of mouse kidney and rat kidney tissue lysate using GTX31145 RhoA antibody [1B8-1C7].
Dilution : 2 µg/ml