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RhoA antibody [1B8-1C7]

Anti-RhoA antibody [1B8-1C7] used in Western Blot (WB). GTX31145
Anti-RhoA antibody [1B8-1C7] used in Western Blot (WB). GTX31145
Anti-RhoA antibody [1B8-1C7] used in Western Blot (WB). GTX31145

Cat. No. GTX31145

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1B8-1C7

Isotype

 IgG1

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all RhoA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Product Note

This antibody detects recombinant RhoA, but not recombinant RhoB or recombinant RhoC.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length recombinant His-tagged human RhoA

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_2887596

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ras homolog family member A , ARH12 , ARHA , RHO12 , RHOH12

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-RhoA antibody [1B8-1C7] used in Western Blot (WB). GTX31145

GTX31145 WB Image

WB analysis of whole cell extracts (30 μg lysate) of Mouse Kidney (Lane 1), HeLa (Lane 2), NIH/3T3 (Lane 3), A-431 (Lane 4), PC-12 (Lane 5), Jurkat (Lane 6), and HEK-293 (Lane 7) using GTX31145 RhoA antibody [1B8-1C7].
Dilution : 1:500-1:2000

Anti-RhoA antibody [1B8-1C7] used in Western Blot (WB). GTX31145

GTX31145 WB Image

WB analysis of human recombinant RhoA-GST (Lane 1), RhoB-GST (Lane 2) and RhoC-GST (Lane 3) using GTX31145 RhoA antibody [1B8-1C7].
Dilution : 2 μg/ml
Loading : 100 ng

Anti-RhoA antibody [1B8-1C7] used in Western Blot (WB). GTX31145

GTX31145 WB Image

WB analysis of 40 ug of mouse kidney and rat kidney tissue lysate using GTX31145 RhoA antibody [1B8-1C7].
Dilution : 2 μg/ml

REFERENCE

Application Reference

Jung HJ et al. Anticancer Res 2019; 39 (11) : 6165-6173 Effect of Tissue Transglutaminase on Steroid Resistance in T-Cell Acute Lymphoblastic Leukemia.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399