Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with RhoB antibody (GTX108600) diluted at 1:1000.

RhoB antibody detects RHOB protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
RhoB antibody (GTX108600) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: U87-MG
12% SDS PAGE
GTX108600 diluted at 1:500

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX108600 diluted at 1:1000