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RhoC antibody

Anti-RhoC antibody used in Western Blot (WB). GTX100546
Anti-RhoC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100546
Anti-RhoC antibody used in IHC (Paraffin sections) (IHC-P). GTX100546
Anti-RhoC antibody used in Western Blot (WB). GTX100546

Cat. No. GTX100546

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all RhoC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

A431 , Mouse liver

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RhoC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721133

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ras homolog family member C , ARH9 , ARHC , H9 , RHOH9

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side

Background

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RhoC antibody used in Western Blot (WB). GTX100546

GTX100546 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX100546 diluted at 1:3000

Anti-RhoC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100546

GTX100546 ICC/IF Image

RhoC antibody detects RHOC protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: RHOC protein stained by RhoC antibody (GTX100546) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-RhoC antibody used in IHC (Paraffin sections) (IHC-P). GTX100546

GTX100546 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma, using RhoC(GTX100546) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RhoC antibody used in Western Blot (WB). GTX100546

GTX100546 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
12% SDS PAGE
GTX100546 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169