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RhoC antibody

KO/KD
Anti-RhoC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55782
Anti-RhoC antibody used in Western Blot (WB). GTX55782
Anti-RhoC antibody used in Western Blot (WB). GTX55782

Cat. No. GTX55782

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Rat
Package
100 μl ($399)
See all RhoC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RHOC (NP_001036144.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ras homolog family member C , ARH9 , ARHC , H9 , RHOH9

Cellular Localization

Cell membrane

Background

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RhoC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55782

GTX55782 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX55782 RhoC antibody.
Blue : DAPI

Anti-RhoC antibody used in Western Blot (WB). GTX55782

GTX55782 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX55782 RhoC antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-RhoC antibody used in Western Blot (WB). GTX55782

GTX55782 WB Image

WB analysis of various sample lysates using GTX55782 RhoC antibody.
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399