Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000 - 1:10000 |
1:20000 - 1:30000 |
Assay dependent |
1:1000 - 1:10000 |
Not tested in other applications.
Product Note
RHODAMINE Monoclonal Antibody was protein A purified and reacts specifically with Rhodamine and its derivatives. Rhodamine isomer 5 and isomer 6 are reactive as TAMRA, as well as TRITC conjugated proteins. No reaction is observed against Texas Red.
Form
Liquid
Buffer
0.02M Potassium Phosphate, 0.15M NaCl
Preservative
0.01% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Anti-RHODAMINE Monoclonal Antibody was produced after repeated immunizations of balb/c mice with rhodamine conjugated KLH.
Purification
Protein A purified
Conjugation
Unconjugated
RRID
AB_385442
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Background
RHODAMINE Monoclonal Antibody specifically detect dyes in the Rhodamine family. Rhodamine is a family member of the fluorone dyes. Examples are Rhodamine 6G and Rhodamine B. They are often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.