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Rhodopsin antibody [1D4]

Anti-Rhodopsin antibody [1D4] used in IHC (Paraffin sections) (IHC-P). GTX25417
Anti-Rhodopsin antibody [1D4] used in IHC (Paraffin sections) (IHC-P). GTX25417
Anti-Rhodopsin antibody [1D4] used in IHC (Paraffin sections) (IHC-P). GTX25417
Anti-Rhodopsin antibody [1D4] used in IHC (Paraffin sections) (IHC-P). GTX25417
Anti-Rhodopsin antibody [1D4] used in Western Blot (WB). GTX25417

Cat. No. GTX25417

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1D4

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP, ELISA

Reactivity

 Human, Mouse, Rat, Bovine
Package
100 μl ($399)
See all Rhodopsin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:1000
ICC/IF 1:10-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Product Note

The epitope for this antibody has been localized to the C-terminal nine amino acids of bovine rhodopsin known as the 1D4 epitope.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Bovine rhodopsin.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

rhodopsin

Cellular Localization

Membrane

Database

Research Area

DATA IMAGES

Anti-Rhodopsin antibody [1D4] used in IHC (Paraffin sections) (IHC-P). GTX25417

GTX25417 IHC-P Image

IHC-P analysis of mouse retinal tissue using GTX25417 Rhodopsin antibody [1D4].
Right : Primary antibody
Left : Negative control without primary antibody
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50

Anti-Rhodopsin antibody [1D4] used in IHC (Paraffin sections) (IHC-P). GTX25417

GTX25417 IHC-P Image

IHC-P analysis of human retinal tissue using GTX25417 Rhodopsin antibody [1D4].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

Anti-Rhodopsin antibody [1D4] used in IHC (Paraffin sections) (IHC-P). GTX25417

GTX25417 IHC-P Image

IHC-P analysis of human retinal tissue using GTX25417 Rhodopsin antibody [1D4].
Right : Primary antibody
Left : Negative control without primary antibody
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50

Anti-Rhodopsin antibody [1D4] used in IHC (Paraffin sections) (IHC-P). GTX25417

GTX25417 IHC-P Image

IHC-P analysis of mouse retinal tissue using GTX25417 Rhodopsin antibody [1D4].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:1000

Anti-Rhodopsin antibody [1D4] used in Western Blot (WB). GTX25417

GTX25417 WB Image

WB analysis of 25 ug of HL60 cell lysates using GTX25417 Rhodopsin antibody [1D4].
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399