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Rhodopsin antibody

Anti-Rhodopsin antibody used in IHC (Frozen sections) (IHC-Fr). GTX129910
Anti-Rhodopsin antibody used in IHC (Paraffin sections) (IHC-P). GTX129910
Anti-Rhodopsin antibody used in Western Blot (WB). GTX129910
Anti-Rhodopsin antibody used in Western Blot (WB). GTX129910

Cat. No. GTX129910

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Rhodopsin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

mouse eye

Predict Reactivity

Human, Rabbit, Bovine, Cat, Dog, Chicken, Guinea pig, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Rhodopsin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886122

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

rhodopsin , CSNBAD1 , OPN2 , RP4

Cellular Localization

Membrane; Multi-pass membrane protein

Background

Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Rhodopsin antibody used in IHC (Frozen sections) (IHC-Fr). GTX129910

GTX129910 IHC-Fr Image

Rhodopsin antibody detects Rhodopsin protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Rhodopsin protein stained by Rhodopsin antibody (GTX129910) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-Rhodopsin antibody used in IHC (Paraffin sections) (IHC-P). GTX129910

GTX129910 IHC-P Image

Rhodopsin antibody detects Rhodopsin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: Rhodopsin stained by Rhodopsin antibody (GTX129910) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rhodopsin antibody used in Western Blot (WB). GTX129910

GTX129910 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody (GTX129910) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Rhodopsin antibody used in Western Blot (WB). GTX129910

GTX129910 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody (GTX129910) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Haggerty KN et al. PLoS Biol 2024; 22 (1) : e3002467 Super-resolution mapping in rod photoreceptors identifies rhodopsin trafficking through the inner segment plasma membrane as an essential subcellular pathway.
Haggerty KN et al. bioRxiv 2023; Mapping rhodopsin trafficking in rod photoreceptors with quantitative super-resolution microscopy.
Pensieri P et al. eNeuro 2021; 8 (5) Photoreceptor cKO of OTX2 Enhances OTX2 Intercellular Transfer in the Retina and Causes Photophobia.
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169
Currently in production