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Rhodopsin antibody

Anti-Rhodopsin antibody used in IHC (Frozen sections) (IHC-Fr). GTX129910
Anti-Rhodopsin antibody used in IHC (Paraffin sections) (IHC-P). GTX129910
Anti-Rhodopsin antibody used in Western Blot (WB). GTX129910
Anti-Rhodopsin antibody used in Western Blot (WB). GTX129910

Cat No. GTX129910

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC-Fr

Reactivity

Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

mouse eye , rat eye

Predict Reactivity

Human, Rabbit, Cat, Dog, Guinea pig, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Rhodopsin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

rhodopsin , CSNBAD1 , OPN2 , RP4

Cellular Localization

Membrane; Multi-pass membrane protein

Background

Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Rhodopsin antibody used in IHC (Frozen sections) (IHC-Fr). GTX129910

GTX129910 IHC-Fr Image

Rhodopsin antibody detects Rhodopsin protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Rhodopsin protein stained by Rhodopsin antibody (GTX129910) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Rhodopsin antibody used in IHC (Paraffin sections) (IHC-P). GTX129910

GTX129910 IHC-P Image

Rhodopsin antibody detects Rhodopsin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: Rhodopsin stained by Rhodopsin antibody (GTX129910) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rhodopsin antibody used in Western Blot (WB). GTX129910

GTX129910 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody (GTX129910) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Rhodopsin antibody used in Western Blot (WB). GTX129910

GTX129910 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody (GTX129910) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169