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Rhodopsin antibody

Anti-Rhodopsin antibody used in IHC (Paraffin sections) (IHC-P). GTX134136
Anti-Rhodopsin antibody used in Western Blot (WB). GTX134136

Cat. No. GTX134136

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse
Package
100 μl ($399),
25 μl ($169)
See all Rhodopsin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

mouse eye

Predict Reactivity

Human, Rat, Rabbit, Bovine, Cat, Dog, Guinea pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of mouse Rhodopsin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887229

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

rhodopsin , Noerg1 , Opn2 , Ops , RP4

Cellular Localization

Membrane

Database

Research Area

DATA IMAGES

Anti-Rhodopsin antibody used in IHC (Paraffin sections) (IHC-P). GTX134136

GTX134136 IHC-P Image

Rhodopsin antibody detects Rhodopsin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: Rhodopsin stained by Rhodopsin antibody (GTX134136) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a cytoplasm marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Rhodopsin antibody used in Western Blot (WB). GTX134136

GTX134136 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Rhodopsin antibody (GTX134136) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169