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Rhotekin antibody

Overexpression
Anti-Rhotekin antibody used in Western Blot (WB). GTX114161
Anti-Rhotekin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114161
Anti-Rhotekin antibody used in Western Blot (WB). GTX114161
Anti-Rhotekin antibody used in Western Blot (WB). GTX114161
Anti-Rhotekin antibody used in Western Blot (WB). GTX114161

Cat. No. GTX114161

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Rhotekin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000

Note :

ICC/IF
suggest to fix with methanol, not recommend to fix with PFA
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

Mouse brain , Jurkat , Raji , NCI-H929 , human Rhotekin-transfected 293T cells

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Rhotekin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619542

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

rhotekin

Background

This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Rhotekin antibody used in Western Blot (WB). GTX114161

GTX114161 WB Image

Sample (20 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX114161 diluted at 1:10000

Anti-Rhotekin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114161

GTX114161 ICC/IF Image

Rhotekin antibody detects Rhotekin protein at cytoplasm by immunofluorescent analysis.
Sample: Rhotekin-transfected (right) or untransfected (left) HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Rhotekin protein stained by Rhotekin antibody (GTX114161) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Rhotekin antibody used in Western Blot (WB). GTX114161

GTX114161 WB Image

Sample (30 ug of whole cell lysate)
A: JurKat
7.5% SDS PAGE
Rhotekin antibody
GTX114161 diluted at 1:1000

Anti-Rhotekin antibody used in Western Blot (WB). GTX114161

GTX114161 WB Image

Overexpression

Rhotekin antibody detects Rhotekin protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human Rhotekin-transfected 293T cells
7.5 % SDS-PAGE
Rhotekin antibody (GTX114161) dilution: 1:5000

Anti-Rhotekin antibody used in Western Blot (WB). GTX114161

GTX114161 WB Image

Rhotekin antibody detects Rhotekin protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
7.5 % SDS-PAGE
Rhotekin antibody (GTX114161) dilution: 1:5000

REFERENCE

Application Reference

Chang YW et al. Cell Cycle 2017; : 0 Midbody localization of Vinexin recruits Rhotekin to facilitate cytokinetic abscission.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169