Western blot analysis of Rhp51 in the whole cell extracts. M: Molecular size markers (kD) Lane 1: Wild-type strain Lane2: Rhp51 deletion mutant strain

Rhp51 foci formation observed after DNA damage S. pombe cells without or with MMS (0.025%) treatment for 1 h were processed for indirect immunofluorescence staining with anti-Rhp51 antibody (1/500 dilution).