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Ribonuclease A antibody

Anti-Ribonuclease A antibody used in Western Blot (WB). GTX105623
Anti-Ribonuclease A antibody used in IHC (Paraffin sections) (IHC-P). GTX105623
Anti-Ribonuclease A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105623

Cat No. GTX105623

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Ribonuclease A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ribonuclease A family member 1, pancreatic , RAC1 , RIB1 , RNS1

Cellular Localization

Secreted

Background

This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq]

Database

DATA IMAGES
Anti-Ribonuclease A antibody used in Western Blot (WB). GTX105623

GTX105623 WB Image

Sample (30 µg of whole cell lysate)
A: U87-MG
12% SDS PAGE
GTX105623 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Ribonuclease A antibody used in IHC (Paraffin sections) (IHC-P). GTX105623

GTX105623 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using Ribonuclease A(GTX105623) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Ribonuclease A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105623

GTX105623 ICC/IF Image

Ribonuclease A antibody detects RNASE1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RNASE1 protein stained by Ribonuclease A antibody (GTX105623) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169