Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ribonuclease A antibody (GTX105623) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Ribonuclease A antibody detects RNASE1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RNASE1 protein stained by Ribonuclease A antibody (GTX105623) diluted at 1:500.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using Ribonuclease A(GTX105623) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min