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Ribonuclease Inhibitor antibody [AT1H23]

Anti-Ribonuclease Inhibitor antibody [AT1H23] used in Western Blot (WB). GTX50017

Cat No. GTX50017

Host

Mouse

Clonality

Monoclonal

Clone Name

AT1H23

Isotype

IgG2a

Application

WB, ELISA

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1000 ~ 2000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Ribonuclease Inhibitor (7-461aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Ribonuclease/Angiogenin Inhibitor 1 , Rai , Rnh , Rnh1

Background

Ribonuclease inhibitor 1(RNH1) the placental ribonuclease inhibitor, is an acid protein with a molecular weight of 50 kDa. RNH1 is a member of a family of proteinaceous cytoplasmic RNase inhibitors that are expressed in many tissues and bind to both intracellular and extracellular RNases in the cytosol. RNH1 is a role in abolishing the ribonucleolytic and angiogenic activities of angiogenin.

Database

Research Area

DATA IMAGES

Anti-Ribonuclease Inhibitor antibody [AT1H23] used in Western Blot (WB). GTX50017

GTX50017 WB Image

The extracts of mouse liver (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human RNH1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf