Mouse tissue extracts (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Ribonuclease T2 antibody [N1C3] (GTX122679) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
GTX122679 diluted at 1:500