GeneTex
United States (US)

Ribophorin I antibody [C2C3], C-term

GTX101888_39631_IFA_19011506_565.jpg
GTX101888_39631_20141128_IHC_19011506_431.jpg
GTX101888_WB_19011506_845.jpg

Cat No. GTX101888

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

H1299 , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Ribophorin I. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp686B16177 antibody, OST1 antibody, RBPH1 antibody, RPN1 antibody, oligosaccharyltransferase 1 homolog antibody, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 antibody, ribophorin-1 antibody, RPN-I antibody, dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit antibody, dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit antibody, ribophorin I antibody

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type I membrane protein , Melanosome

Background

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq]

Database

DATA IMAGES
GTX101888_39631_IFA_19011506_565.jpg

GTX101888 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Ribophorin I(GTX101888) antibody at 1:500 dilution.

GTX101888_39631_20141128_IHC_19011506_431.jpg

GTX101888 IHC-P Image

Ribophorin I antibody [C2C3], C-term detects Ribophorin I protein at cytoplasm on human normal kidney by immunohistochemical analysis.
Sample: Paraffin-embedded human normal kidney.
Ribophorin I antibody [C2C3], C-term (GTX101888) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101888_WB_19011506_845.jpg

GTX101888 WB Image

Sample(30 μg of whole cell lysate)
A:H1299
B:Hep G2(GTX27900)
10% SDS PAGE
GTX101888 diluted at 1:1500

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169