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Ribophorin I antibody [C2C3], C-term

Anti-Ribophorin I antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101888
Anti-Ribophorin I antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101888
Anti-Ribophorin I antibody [C2C3], C-term used in Western Blot (WB). GTX101888

Cat No. GTX101888

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

H1299 , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Ribophorin I. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ribophorin I , OST1 , RBPH1

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type I membrane protein , Melanosome

Background

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Ribophorin I antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101888

GTX101888 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Ribophorin I(GTX101888) antibody at 1:500 dilution.

Anti-Ribophorin I antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101888

GTX101888 IHC-P Image

Ribophorin I antibody [C2C3], C-term detects Ribophorin I protein at cytoplasm on human normal kidney by immunohistochemical analysis.
Sample: Paraffin-embedded human normal kidney.
Ribophorin I antibody [C2C3], C-term (GTX101888) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Ribophorin I antibody [C2C3], C-term used in Western Blot (WB). GTX101888

GTX101888 WB Image

Sample(30 μg of whole cell lysate)
A:H1299
B:Hep G2(GTX27900)
10% SDS PAGE
GTX101888 diluted at 1:1500

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169