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Ribophorin I antibody [C2C3], C-term

Anti-Ribophorin I antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101888
Anti-Ribophorin I antibody [C2C3], C-term used in Western Blot (WB). GTX101888
Anti-Ribophorin I antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101888

Cat. No. GTX101888

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Ribophorin I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

H1299 , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Ribophorin I. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951761

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribophorin I , OST1 , RBPH1

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type I membrane protein , Melanosome

Background

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Ribophorin I antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101888

GTX101888 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Ribophorin I(GTX101888) antibody at 1:500 dilution.

Anti-Ribophorin I antibody [C2C3], C-term used in Western Blot (WB). GTX101888

GTX101888 WB Image

Sample(30 μg of whole cell lysate)
A:H1299
B:Hep G2(GTX27900)
10% SDS PAGE
GTX101888 diluted at 1:1500

Anti-Ribophorin I antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101888

GTX101888 IHC-P Image

Ribophorin I antibody [C2C3], C-term detects Ribophorin I protein at cytoplasm on human normal kidney by immunohistochemical analysis.
Sample: Paraffin-embedded human normal kidney.
Ribophorin I antibody [C2C3], C-term (GTX101888) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169