GeneTex
United States (US)

Ribosome binding protein 1 antibody [C3], C-term

GTX101844_39596_IFA_2_19011506_801.jpg
GTX101844_39596_IFA_19011506_991.jpg
GTX101844_WB_M_19011506_435.jpg
GTX101844_IHC_19011506_746.jpg
GTX101844_39596_WB_siRNA_19011506_157.jpg

Cat No. GTX101844

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

152 kDa. ( Note )

Positive Control

HepG2 , NIH-3T3

Predict Reactivity

Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Ribosome binding protein 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ribosome Binding Protein 1,Es/130,Es130,Rrp,Hes,Rrbp1

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type III membrane protein

Background

Analysis of cDNA clones indicates that ribosome binding protein 1 may exist in different forms due to removal of tandem repeats, or partial intraexonic splicing of RRBP1. The form presented here is lacking the canine p180 ribosome-binding domain, NQGKKAEGAQ, which is tandemly repeated close to the N-terminus in other forms that haven't been fully characterized. RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq]

Database

DATA IMAGES
GTX101844_39596_IFA_2_19011506_801.jpg

GTX101844 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Ribosome binding protein 1(GTX101844) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

GTX101844_39596_IFA_19011506_991.jpg

GTX101844 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Ribosome binding protein 1(GTX101844) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

GTX101844_WB_M_19011506_435.jpg

GTX101844 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3
5% SDS PAGE
GTX101844 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101844_IHC_19011506_746.jpg

GTX101844 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using Ribosome binding protein 1(GTX101844) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101844_39596_WB_siRNA_19011506_157.jpg

GTX101844 WB Image

Ribosome binding protein 1 antibody validation by siRNA knock-down.
Upperpanel: Ribosome binding protein 1 antibody GTX101844
Lower panel: Vinculin antibody (GTX109749)
A. 30 µg HepG2 whole cell lysate/extract
B. 30 µg whole cell lysate/extract of RRBP1 siRNA#1-transfected HepG2 cells
C. 30 µg whole cell lysate/extract of RRBP1 siRNA#2-transfected HepG2 cells
5% SDS-PAGE
Ribosome binding protein 1 antibody (GTX101844) dilution: 1:500
Vinculin antibody (GTX109749) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169