Ribosome binding protein 1 antibody [GT5610]

Cat. No. GTX628998

Host	Mouse
Clonality	Monoclonal
Clone Name	GT5610
Isotype	IgG2a
Application	WB, ICC/IF
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

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Datasheet
Document

View product citations for antibody GTX628998 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:2000

Not tested in other applications.

Calculated MW

152 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Ribosome binding protein 1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888086

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosome binding protein 1 , ES/130 , ES130 , RRp , hES

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type III membrane protein

Background

Analysis of cDNA clones indicates that ribosome binding protein 1 may exist in different forms due to removal of tandem repeats, or partial intraexonic splicing of RRBP1. The form presented here is lacking the canine p180 ribosome-binding domain, NQGKKAEGAQ, which is tandemly repeated close to the N-terminus in other forms that haven't been fully characterized. RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq]

Database

Gene ID: 6238 RRBP1
UniProt: Q9P2E9 RRBP1

Research Area

DATA IMAGES

GTX628998 WB Image

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Ribosome binding protein 1 antibody [GT5610] (GTX628998) diluted at 1:500.

GTX628998 ICC/IF Image

Ribosome binding protein 1 antibody [GT5610] detects Ribosome binding protein 1 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ribosome binding protein 1 protein stained by Ribosome binding protein 1 antibody [GT5610] (GTX628998) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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SDS
PBS.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations