GeneTex
United States (US)

Robo1 antibody

GTX36928_20160330_IHC-Fr_18121410_159.jpg
GTX36928_20160330_WB_18121410_797.jpg
GTX36928_20160330_IHC-P_18121410_276.jpg

Cat No. GTX36928

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, ELISA
Reactivity Human, Mouse, Rat, Dog
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:3000
ICC/IF Assay dependent
IHC-P 2 μg/ml-10 μg/ml
IHC-Fr 2 μg/ml to 10 μg/ml
ELISA 1:30000 - 1:60000
Not tested in other applications.

Calculated MW

181 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to amino acids 1632-1644 of Human Robo1

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Roundabout Guidance Receptor 1,Dutt1,Sax3,Robo1

Background

ROBO-1 (also called Roundabout homolog 1 precursor and Deleted in U twenty twenty (DUTT)) functions as a receptor for SLIT1 and SLIT2. The SLIT proteins are thought to act as a molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. ROBO-1 may also be required for lung development. ROBO-1 is a type I membrane protein. ROBO-1 is a widely expressed protein with the exception of the kidney. Defects in ROBO1 may be a cause of breast and lung cancer. ROBO-1 maps within a region of overlapping homozygous deletions characterized in both small cell lung cancer cell lines (SCLC) and in a breast cancer cell line. Multiple splice variants have been identified for this protein.

Database

Research Area

DATA IMAGES
GTX36928_20160330_IHC-Fr_18121410_159.jpg

GTX36928 IHC-Fr Image

1/50 staining mouse lung tissue sections (adult, frozen 100µm wholemount sections) by IHC-Fr. The tissue was paraformaldehyde fixed and permeabilized with polyethylene glycol tert-octylphenyl ether before incubation with the antibody (GTX36928) for 16 hours at 4°C.

GTX36928_20160330_WB_18121410_797.jpg

GTX36928 WB Image

Western blot using Affinity Purified anti-ROBO-1 antibody (GTX36928) shows detection of a band at ~181 kDa corresponding to ROBO-1 present in mouse brain lysate (arrowhead). Approximately 35 µg of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of infrared 800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. Infrared 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.

GTX36928_20160330_IHC-P_18121410_276.jpg

GTX36928 IHC-P Image

Affinity Purified anti-ROBO1 antibody (GTX36928) was used at a concentration of 5 µg/ml to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows staining of human brain tissue. Tissue was formalin-fixed and paraffin embedded.

Package List Price ($)
$ 359