WB analysis of various Arabidopsis extracts grown under 0.2 mM nitrate for 7 days using GTX00909 Root FNR antibody. R-FNR2 is dominant form in wild-type roots. Wild type produces both R-FNR1 and R-FNR2. Mutant rfnr1 produces R-FNR2 and mutant rfr2 produces R-FNR1.
Lane 1 & 3 : wild type strain Arabidopsis Col
Lane 2 : mutant rfnr1
Lane 4 : rfnr2-2
Dilution : 1:2000