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Rpl3 antibody

Anti-Rpl3 antibody used in Western Blot (WB). GTX124464
Anti-Rpl3 antibody used in IHC (Whole mount) (IHC-Wm). GTX124464
Anti-Rpl3 antibody used in IHC (Paraffin sections) (IHC-P). GTX124464

Cat. No. GTX124464

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Rpl3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm 1:100-1:500
Not tested in other applications.

Positive Control

whole zebrafish

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Rpl3

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169700

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein L3 , wu:fa99g02 , wu:fb65e09 , zgc:110350

Database

Research Area

DATA IMAGES

Anti-Rpl3 antibody used in Western Blot (WB). GTX124464

GTX124464 WB Image

Sample (30 ug of whole cell lysate)
A: whole zebrafish
10% SDS PAGE
GTX124464 diluted at 1:1000

Anti-Rpl3 antibody used in IHC (Whole mount) (IHC-Wm). GTX124464

GTX124464 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using rpl3 (GTX124464) antibody at 1:200 dilution.

Anti-Rpl3 antibody used in IHC (Paraffin sections) (IHC-P). GTX124464

GTX124464 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using rpl3 (GTX124464) antibody at 1:300 dilution.

REFERENCE

Application Reference

Leiendecker L et al. Cancer Discov 2023; 13 (1) : 70-84 Human Papillomavirus 42 Drives Digital Papillary Adenocarcinoma and Elicits a Germ Cell-like Program Conserved in HPV-Positive Cancers.
Leesch F et al. Nature 2023; 613 (7945) : 712-720 A molecular network of conserved factors keeps ribosomes dormant in the egg.
Application : WB
Reactivity : Zebrafish
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169