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Rpsa antibody

Anti-Rpsa antibody used in Western Blot (WB). GTX124343
Anti-Rpsa antibody used in IHC (Paraffin sections) (IHC-P). GTX124343

Cat No. GTX124343

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse, Zebrafish
Package
200 μl ($409),
50 μl ($219)
See all zebrafish Rpsa products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

Adult zebrafish , Zebrafish eye , Ubiquitous express

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Rpsa

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ribosomal protein SA , lamr1 , zgc:55831 , zgc:77824

Cellular Localization

Cell membrane , Cytoplasm , Nucleus

Background

Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis.

Database

Research Area

DATA IMAGES

Anti-Rpsa antibody used in Western Blot (WB). GTX124343

GTX124343 WB Image

Sample (30 ug of whole cell lysate)
A: whole zebrafish
B: zebrafish eye
12% SDS PAGE
GTX124343 diluted at 1:1000

Anti-Rpsa antibody used in IHC (Paraffin sections) (IHC-P). GTX124343

GTX124343 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using rpsa (GTX124343) antibody at 1:300 dilution.

REFERENCE

Application Reference

Heisler FF et al. Neuron 2018; 99 (6) : 1155-1169.e9 Muskelin Coordinates PrPC Lysosome versus Exosome Targeting and Impacts Prion Disease Progression.
Application : WB
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 409
$ 219