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Rpsa antibody

Anti-Rpsa antibody used in Western Blot (WB). GTX124343
Anti-Rpsa antibody used in IHC (Paraffin sections) (IHC-P). GTX124343

Cat No. GTX124343

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse, Zebrafish
Package
200 μl ($409),
50 μl ($219)
See all zebrafish Rpsa products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

Adult zebrafish , Zebrafish eye , Ubiquitous express

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Rpsa

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169369

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ribosomal protein SA , lamr1 , zgc:55831 , zgc:77824

Cellular Localization

Cell membrane , Cytoplasm , Nucleus

Background

Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis.

Database

Research Area

DATA IMAGES

Anti-Rpsa antibody used in Western Blot (WB). GTX124343

GTX124343 WB Image

Sample (30 ug of whole cell lysate)
A: whole zebrafish
B: zebrafish eye
12% SDS PAGE
GTX124343 diluted at 1:1000

Anti-Rpsa antibody used in IHC (Paraffin sections) (IHC-P). GTX124343

GTX124343 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using rpsa (GTX124343) antibody at 1:300 dilution.

REFERENCE

Application Reference

Heisler FF et al. Neuron 2018; 99 (6) : 1155-1169.e9 Muskelin Coordinates PrPC Lysosome versus Exosome Targeting and Impacts Prion Disease Progression.
Application : WB
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 409
$ 219