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Rub1 antibody

Anti-Rub1 antibody used in Western Blot (WB). GTX48823
Anti-Rub1 antibody used in Western Blot (WB). GTX48823

Cat. No. GTX48823

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Yeast
Package
100 μg ($399)
See all Rub1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ELISA 1:1000-1:5000
Not tested in other applications.

Calculated MW

9 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Yeast

Immunogen

Full length recombinant yeast Rub1 protein.

Purification

IgG fraction
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

NEDD8 family protein RUB1

Database

Research Area

DATA IMAGES

Anti-Rub1 antibody used in Western Blot (WB). GTX48823

GTX48823 WB Image

Anti-Rub1 antibody, generated by immunization with full-length, recombinant yeast Rub1, was tested by western blot against a yeast cell lysate. A dilution of the antibody between 1:200 and 1:1,000 will show strong reactivity specifically with free Rub1 protein (indicated by arrow) and Rub1 conjugates. In this blot, the antibody was used at a 1:500 dilution and was incubated overnight at 4º C in 5% non-fat dry milk in TBST. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG

Anti-Rub1 antibody used in Western Blot (WB). GTX48823

GTX48823 WB Image

WB analysis of recombinant yeast Rub1 protein using GTX48823 Rub1 antibody.
Dilution : 1:500

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399