GeneTex
  • Country / Location Selection

United States (US)

Rub1 antibody

Anti-Rub1 antibody used in Western Blot (WB). GTX48823

Cat No. GTX48823

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Yeast
Package
100 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2,000
ELISA 1:1,000 - 1:5,000
Not tested in other applications.

Calculated MW

9 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Yeast

Immunogen

Rub1 Antibody was prepared from rabbit serum after repeated immunizations with full-length recombinant yeast Rub1 protein.

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

NEDD8 family protein RUB1

Database

DATA IMAGES

Anti-Rub1 antibody used in Western Blot (WB). GTX48823

GTX48823 WB Image

Anti-Rub1 antibody, generated by immunization with full-length, recombinant yeast Rub1, was tested by western blot against a yeast cell lysate. A dilution of the antibody between 1:200 and 1:1,000 will show strong reactivity specifically with free Rub1 protein (indicated by arrow) and Rub1 conjugates. In this blot, the antibody was used at a 1:500 dilution and was incubated overnight at 4º C in 5% non-fat dry milk in TBST. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG

REFERENCE

There are currently no references for Rub1 antibody (GTX48823). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Rub1 antibody (GTX48823). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 319