Rubicon antibody immunoprecipitates Rubicon protein in IP experiments.
IP samples: whole cell extract of Rubicon-transfected 293T cells
A. 30 μg 293T whole cell extract of Rubicon-protein expressing cell
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of Rubicon protein by 4 μg Rubicon antibody (GTX129096)
5 % SDS-PAGE
The immunoprecipitated Rubicon protein was detected by Rubicon antibody (GTX129096) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Rubicon antibody detects Rubicon protein by western blot analysis.
A. 30 μg MDA-MB-231 whole cell extract
5 % SDS-PAGE
Rubicon antibody (GTX129096) dilution: 1:1000

Rubicon antibody detects Rubicon protein by western blot analysis.
A. 30 ug 293T whole cell extract
B. 30 whole cell extract of human Rubicon-transfected 293T cells
5 % SDS-PAGE
Rubicon antibody (GTX129096) dilution: 1:20000

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Rubicon antibody (GTX129096) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.