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Rubicon antibody

Anti-Rubicon antibody used in Western Blot (WB). GTX129096
Anti-Rubicon antibody used in Western Blot (WB). GTX129096
Anti-Rubicon antibody used in Immunoprecipitation (IP). GTX129096
Anti-Rubicon antibody used in Western Blot (WB). GTX129096

Cat No. GTX129096

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

109 kDa. ( Note )

Positive Control

MDA-MB-231 , NT2D1 , human Rubicon-transfected 293T

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Rubicon. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

rubicon autophagy regulator , KIAA0226 , RUBICON , SCAR15

Background

Negatively regulates endocytic trafficking. Impairs the autophagosome maturation process. May inhibit PIK3C3 activity.

Database

Research Area

DATA IMAGES

Anti-Rubicon antibody used in Western Blot (WB). GTX129096

GTX129096 WB Image

Rubicon antibody detects Rubicon protein by western blot analysis.
A. 30 μg NT2D1 whole cell extract
5 % SDS-PAGE
Rubicon antibody (GTX129096) dilution: 1:1000

Anti-Rubicon antibody used in Western Blot (WB). GTX129096

GTX129096 WB Image

Rubicon antibody detects Rubicon protein by western blot analysis.
A. 30 ug 293T whole cell extract
B. 30 whole cell extract of human Rubicon-transfected 293T cells
5 % SDS-PAGE
Rubicon antibody (GTX129096) dilution: 1:20000

Anti-Rubicon antibody used in Immunoprecipitation (IP). GTX129096

GTX129096 IP Image

Rubicon antibody immunoprecipitates Rubicon protein in IP experiments.
IP samples: whole cell extract of Rubicon-transfected 293T cells
A. 30 μg 293T whole cell extract of Rubicon-protein expressing cell
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of Rubicon protein by 4 μg Rubicon antibody (GTX129096)
5 % SDS-PAGE
The immunoprecipitated Rubicon protein was detected by Rubicon antibody (GTX129096) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-Rubicon antibody used in Western Blot (WB). GTX129096

GTX129096 WB Image

Rubicon antibody detects Rubicon protein by western blot analysis.
A. 30 μg MDA-MB-231 whole cell extract
5 % SDS-PAGE
Rubicon antibody (GTX129096) dilution: 1:1000

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169