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RyR2 (phospho Ser2814) antibody

Anti-RyR2 (phospho Ser2814) antibody used in IHC (Paraffin sections) (IHC-P). GTX00626
Anti-RyR2 (phospho Ser2814) antibody used in ELISA (ELISA). GTX00626
Anti-RyR2 (phospho Ser2814) antibody used in Western Blot (WB). GTX00626

Cat No. GTX00626

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
IHC-P 1:50-1:200
ELISA Assay dependent
Not tested in other applications.

Calculated MW

565 kDa. ( Note )

Predict Reactivity

Rabbit, Sheep, Bovine, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human RYR2 around the phosphorylation site of Ser2814.

Purification

Purified by sequential chromatography on phospho-peptide and non-phospho-peptide columns

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ryanodine receptor 2 , ARVC2 , ARVD2 , RYR-2 , RyR , VTSIP

Cellular Localization

Membrane

Background

This gene encodes a ryanodine receptor found in cardiac muscle sarcoplasmic reticulum. The encoded protein is one of the components of a calcium channel, composed of a tetramer of the ryanodine receptor proteins and a tetramer of FK506 binding protein 1B proteins, that supplies calcium to cardiac muscle. Mutations in this gene are associated with stress-induced polymorphic ventricular tachycardia and arrhythmogenic right ventricular dysplasia. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-RyR2 (phospho Ser2814) antibody used in IHC (Paraffin sections) (IHC-P). GTX00626

GTX00626 IHC-P Image

IHC-P analysis of human thyroid cancer tissue using GTX00626 RyR2 (phospho Ser2814) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

Anti-RyR2 (phospho Ser2814) antibody used in ELISA (ELISA). GTX00626

GTX00626 ELISA Image

ELISA analysis of RyR2 phospho- and non-phospho peptide using GTX00626 RyR2 (phospho Ser2814) antibody.
P-peptide: phospho-peptide
N-peptide: non-phospho-peptide
Peptides concentration : 1μg/ml

Anti-RyR2 (phospho Ser2814) antibody used in Western Blot (WB). GTX00626

GTX00626 WB Image

WB analysis of A431 cell lysate using GTX00626 RyR2 (phospho Ser2814) antibody.
Lane 1 : Primary antibody prior incubate with phospho-blocking peptide
Lane 2 : Primary antibody prior incubate with non-phospho-blocking peptide

REFERENCE

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REVIEW

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Package List Price ($)
$ 319