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S Tag antibody [GT247]

Anti-S Tag antibody [GT247] used in Immunoprecipitation (IP). GTX628791
Anti-S Tag antibody [GT247] used in Western Blot (WB). GTX628791

Cat No. GTX628791

Host

Mouse

Clonality

Monoclonal

Clone Name

GT247

Isotype

IgG2b

Application

WB, IP
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IP 1:500-1:1000
Not tested in other applications.

Positive Control

S-tagged protein expressing 293T cells

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to S Tag

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888053

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-S Tag antibody [GT247] used in Immunoprecipitation (IP). GTX628791

GTX628791 IP Image

S tag antibody immunoprecipitates S-fused protein in IP experiments. IP Sample: 400 μg cell lysate/extract of S-fused gene transfected 293T cells A. 30 μg lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune mouse IgG C. Immunoprecipitation of S-fused protein by 2 μg of S tag antibody (GTX628791) 12% SDS-PAGE The immunoprecipitated S-fused protein was detected by S tag antibody (GTX628791) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-S Tag antibody [GT247] used in Western Blot (WB). GTX628791

GTX628791 WB Image

S Tag antibody detects S protein by western blot analysis.
A. 30 μg 293T whole cell lysate/eztract
B. 30 μg whole cell lysate/extract of S-tagged protein expressing 293T cells
12% SDS-PAGE
S Tag antibody (GTX628791) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 379
$ 169
Custom Conjugations