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S Tag antibody

Anti-S Tag antibody used in Immunoprecipitation (IP). GTX128060
Anti-S Tag antibody used in Western Blot (WB). GTX128060
Anti-S Tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128060

Cat No. GTX128060

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IP 2.5 ug
Not tested in other applications.

Positive Control

S-tag-transfected 293T cells

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.24 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to S Tag.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

S antibody

Research Area

DATA IMAGES

Anti-S Tag antibody used in Immunoprecipitation (IP). GTX128060

GTX128060 IP Image

S-tag antibody immunoprecipitates S-tag protein in IP experiments. IP Sample: 650 μg whole cell lysate/extract of S-tag protein expressing HeLa cells. A. 20 μg whole cell lysate/extract of transfected HeLa cells B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of S-tag protein by 2.5 μg of S-tag antibody (GTX128060) 12% SDS-PAGE The immunoprecipitated S-tag protein was detected by S-tag antibody (GTX128060) diluted at 1:10000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-S Tag antibody used in Western Blot (WB). GTX128060

GTX128060 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with S Tag antibody (GTX128060) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-S Tag antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128060

GTX128060 ICC/IF Image

S Tag antibody detects S protein by immunofluorescent analysis.
Sample: S-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: S stained by S Tag antibody (GTX128060) diluted at 1:2000.
Blue: Hoechst 33342 staining.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169