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S100 alpha 2 antibody [N1C3]

Anti-S100 alpha 2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121457
Anti-S100 alpha 2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX121457
Anti-S100 alpha 2 antibody [N1C3] used in Western Blot (WB). GTX121457

Cat. No. GTX121457

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all S100 alpha 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human S100 alpha 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10724806

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

S100 calcium binding protein A2 , CAN19 , S100L

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may have a tumor suppressor function. Chromosomal rearrangements and altered expression of this gene have been implicated in breast cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-S100 alpha 2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121457

GTX121457 ICC/IF Image

S100A2 antibody [N1C3] detects S100A2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: S100A2 protein stained by S100A2 antibody [N1C3] (GTX121457) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-S100 alpha 2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX121457

GTX121457 IHC-P Image

S100A2 antibody [N1C3] detects S100A2 protein at cytoplasm on human normal kidney by immunohistochemical analysis.
Sample: Paraffin-embedded human normal kidney.
S100A2 antibody [N1C3] (GTX121457) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-S100 alpha 2 antibody [N1C3] used in Western Blot (WB). GTX121457

GTX121457 WB Image

Sample (30 ug of whole cell lysate)
A: A431
15% SDS PAGE
GTX121457 diluted at 1:10000

REFERENCE

Application Reference

Lin SJ et al. J Proteomics 2013; 94 : 186-201 Low-molecular-mass secretome profiling identifies C-C motif chemokine 5 as a potential plasma biomarker and therapeutic target for nasopharyngeal carcinoma.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169