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S100 alpha antibody

Anti-S100 alpha antibody used in Western Blot (WB). GTX11428
Anti-S100 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11428

Cat. No. GTX11428

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ELISA

Reactivity

 Human, Mouse, Rat, Bovine, Pig
Package
50 μg ($399)
See all S100 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1 μg/ml
ICC/IF 1:50-1:200
IHC-P 1 μg/ml
IP 5 μg/ml
ELISA Assay dependent

Note :

WB
S100 protein is relatively small and, therefore, it is recommended that the electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane.
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Product Note

This antibody has shown no cross-reactivity with the S100-beta protein. Immunohistochemical staining on paraffin sections demonstrates that S100-alpha is localized in serous cells, myoepithelial cells and epithelial cells of intercalated ducts in the parotid gland.

PROPERTIES

Form

Liquid

Buffer

PBS, 1.5% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified S100-alpha protein from human pectoral muscle cells.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_372948

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

S100 calcium binding protein A1 , S100 , S100-alpha , S100A

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-S100 alpha antibody used in Western Blot (WB). GTX11428

GTX11428 WB Image

WB analysis of human muscle extract using GTX11428 S100 alpha antibody.

Anti-S100 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11428

GTX11428 ICC/IF Image

ICC/IF analysis of HEK293T cells using GTX11428 S100 alpha antibody.
Fixation : formaldehyde
Permeabilization : 0.3% Trito X-100 for 60 minutes
Dilution : 1:100 overnight at 4 ºC

REFERENCE

Application Reference

Desjardins JF et al. Am J Physiol Heart Circ Physiol 2009; 296 (5) : H1457-65 Lack of S100A1 in mice confers a gender-dependent hypertensive phenotype and increased mortality after myocardial infarction.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399