S100 antibody [8B10]

GTX28330 at a dilution of 1/1000, staining S100 (Alexa 488 secondary at 1/2000) on rat brain tissue (30m thick coronal sections) in free floating IHC (see protocol link for detailed description). Images showing neuron body and processes: [A] 20x objective

Interaction of several monoclonal antibodies available to the S100 protein (1 g) from human brain in WB, after native gel electrophoresis using the Ornstein-Davis system. Lane 1 = GTX14849, Lane 2 = GTX28330, ab Lane 3 = GTX10203, Lane 4 = GTX28334

S-100 calibration curve, from one step assay in streptavidin coated plates. GTX28330 [200 ng/well] was used as capture and Eu- labelled GTX10203 [200 ng/well] as detection. Antigen used was S-100 protein from the human brain. Incubated 20 minutes at 20C.