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S100 antibody [8B10]

Anti-S100 antibody [8B10] used in ELISA (ELISA). GTX28330
Anti-S100 antibody [8B10] used in Western Blot (WB). GTX28330
Anti-S100 antibody [8B10] used in Immunohistochemistry (IHC). GTX28330

Cat. No. GTX28330

Host

Mouse

Clonality

Monoclonal

Clone Name

8B10

Isotype

IgG1

Application

WB, IHC-P, ELISA, EIA, IHC

Reactivity

Human
Package
200 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
EIA Assay dependent
IHC Assay dependent
Not tested in other applications.

Product Note

Specific to S100BB and S100A1B.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length protein (Human).

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-S100 antibody [8B10] used in ELISA (ELISA). GTX28330

GTX28330 ELISA Image

S-100 calibration curve, from one step assay in streptavidin coated plates. GTX28330 [200 ng/well] was used as capture and Eu- labelled GTX10203 [200 ng/well] as detection. Antigen used was S-100 protein from the human brain. Incubated 20 minutes at 20C.

Anti-S100 antibody [8B10] used in Western Blot (WB). GTX28330

GTX28330 WB Image

Interaction of several monoclonal antibodies available to the S100 protein (1 g) from human brain in WB, after native gel electrophoresis using the Ornstein-Davis system. Lane 1 = GTX14849, Lane 2 = GTX28330, ab Lane 3 = GTX10203, Lane 4 = GTX28334

Anti-S100 antibody [8B10] used in Immunohistochemistry (IHC). GTX28330

GTX28330 IHC Image

GTX28330 at a dilution of 1/1000, staining S100 (Alexa 488 secondary at 1/2000) on rat brain tissue (30m thick coronal sections) in free floating IHC (see protocol link for detailed description). Images showing neuron body and processes: [A] 20x objective

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399