GeneTex
United States (US)

S100 antibody [8B10]

S100-antibody-8B10-GTX28330-WB-1_18121411_297.jpg
S100-antibody-8B10-GTX28330-ELISA-1_18121411_931.jpg
S100-antibody-8B10-GTX28330-IHC-1_18121411_284.jpg

Cat No. GTX28330

Host Mouse
Clonality Monoclonal
Clone Name 8B10
Isotype IgG1
Application WB, IHC-P, ELISA, EIA, IHC
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
EIA Assay dependent
IHC Assay dependent
Not tested in other applications.

Specificity/Sensitivity

Specific to S100BB and S100A1B.
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 0.1 % sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4º C.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length protein (Human).

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DATA IMAGES
S100-antibody-8B10-GTX28330-WB-1_18121411_297.jpg

GTX28330 WB Image

Interaction of several monoclonal antibodies available to the S100 protein (1 g) from human brain in WB, after native gel electrophoresis using the Ornstein-Davis system. Lane 1 = GTX14849, Lane 2 = GTX28330, ab Lane 3 = GTX10203, Lane 4 = GTX28334

S100-antibody-8B10-GTX28330-ELISA-1_18121411_931.jpg

GTX28330 ELISA Image

S-100 calibration curve, from one step assay in streptavidin coated plates. GTX28330 [200 ng/well] was used as capture and Eu- labelled GTX10203 [200 ng/well] as detection. Antigen used was S-100 protein from the human brain. Incubated 20 minutes at 20C.

S100-antibody-8B10-GTX28330-IHC-1_18121411_284.jpg

GTX28330 IHC Image

GTX28330 at a dilution of 1/1000, staining S100 (Alexa 488 secondary at 1/2000) on rat brain tissue (30m thick coronal sections) in free floating IHC (see protocol link for detailed description). Images showing neuron body and processes: [A] 20x objective

Package List Price ($)
$ 239