S100 beta antibody [4C4.9]

Cat. No. GTX04511

Host	Mouse
Clonality	Monoclonal
Clone Name	4C4.9
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human, Mouse, Rat, Bovine
Package	100 μg ($529)

See all S100 beta products

Datasheet

View product citations for antibody GTX04511 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2 μg/ml
ICC/IF	1-2 μg/ml
IHC-P	0.25-0.5 μg/ml
FACS	1-2μg/1x10⁶ cells
Note : IHC-P Recommend pretreatment: heating tissue sections in 10mM Tris with 1mM EDTA (pH 9.0)

Not tested in other applications.

Calculated MW

11-12 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA (Please contact us for PBS only format)

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Purified bovine brain S100 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

S100 calcium binding protein B , NEF , S100 , S100-B , S100beta

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]

Database

Gene ID: 6285 S100B
UniProt: P04271 S100B

Research Area

DATA IMAGES

GTX04511 Image

SDS-PAGE analysis of GTX04511 S100 beta antibody [4C4.9].

GTX04511 ICC/IF Image

ICC/IF analysis of A2058 cells using GTX04511 S100 beta antibody [4C4.9].
Green : Primary antibody / Isotype control
Red : F-actin
Blue : DAPI

GTX04511 WB Image

WB analysis of human brain tissue lysates using GTX04511 S100 beta antibody [4C4.9].

GTX04511 IHC-P Image

IHC-P analysis of human melanoma tissue using GTX04511 S100 beta antibody [4C4.9].

REFERENCE

There are currently no references for S100 beta antibody [4C4.9] (GTX04511). Be the first to share your publications with this product.

Submit a Reference

REVIEW

There are currently no reviews for S100 beta antibody [4C4.9] (GTX04511). Be the first to share your experience with this product.

Submit a Review

Package

List Price ($)

100 μg

$ 529

Qty