S100 beta antibody [HL2228]

Whole cell extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with S100 beta antibody [HL2228] (GTX638273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

S100 beta antibody [HL2228] detects S100 beta protein by immunohistochemical analysis.
Sample: Paraffin-embedded human appendix.
S100 beta stained by S100 beta antibody [HL2228] (GTX638273) diluted at 1:32000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

S100 beta antibody [HL2228] detects S100 beta protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tissues.
S100 beta stained by S100 beta antibody [HL2228] (GTX638273) diluted at 1:32000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

S100 beta antibody [HL2228] detects S100 beta protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
S100 beta stained by S100 beta antibody [HL2228] (GTX638273) diluted at 1:25000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

S100 beta antibody [HL2228] detects S100 beta protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
Green: S100 beta stained by S100 beta antibody [HL2228] (GTX638273) diluted at 1:12500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

S100 beta antibody [HL2228] detects S100 beta protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebral cortex.
S100 beta stained by S100 beta antibody [HL2228] (GTX638273) diluted at 1:50000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human S100 beta protein (nM) (46.73-0.73 nM). Coated protein was probed with S100 beta antibody [HL2228] (GTX638273) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

S100 beta antibody [HL2228] detects S100 beta protein by immunohistochemical analysis.
Sample: Paraffin-embedded human glioblastoma.
S100 beta stained by S100 beta antibody [HL2228] (GTX638273) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

S100 beta antibody [HL2228] detects S100 beta protein at glia by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: S100 beta stained by S100 beta antibody [HL2228] (GTX638273) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with S100 beta antibody [HL2228] (GTX638273) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with S100 beta antibody [HL2228] (GTX638273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

S100 beta antibody [HL2228] detects S100 beta protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat hippocampus.
Green: S100 beta stained by S100 beta antibody [HL2228] (GTX638273) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

S100 beta antibody [HL2228] detects S100 beta protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: S100 beta stained by S100 beta antibody [HL2228] (GTX638273) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with S100 beta antibody [HL2228] (GTX638273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.