PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Appendix: Schwann cells of peripheral nerves and adipocytes should show a strong, predominantly cytoplasmic S100B staining.
Negative Control
Appendix: Smooth muscle and epithelial cells must not show any S100B staining.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human S100B protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
S100 calcium binding protein B , NEF , S100 , S100-B , S100beta
Cellular Localization
Cytoplasm , Nucleus
Background
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-S100 beta antibody [MSVA-490R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04404](/templates/default/js/lightbox/images/loading.gif "Anti-S100 beta antibody [MSVA-490R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04404")
|
GTX04404 IHC-P Image
IHC-P analysis of human cerebrum (grey matter) tissue using GTX04404 S100 beta antibody [MSVA-490R] HistoMAX™.
Strong ubiquitous S100 beta staining, except neirons.
|
|
|
GTX04404 IHC-P Image
IHC-P analysis of human appendix tissue using GTX04404 S100 beta antibody [MSVA-490R] HistoMAX™.
In the appendix, Schwann cells of peripheral nerves show strong S100 beta positivity.
|
|
|
GTX04404 IHC-P Image
IHC-P analysis of human malignant melanoma from skin tissue using GTX04404 S100 beta antibody [MSVA-490R] HistoMAX™.
Malignant melanoma with strong S100 beta positivity in all tumor cells.
|
REFERENCE
There are currently no references for S100 beta antibody [MSVA-490R] HistoMAX™ (GTX04404). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for S100 beta antibody [MSVA-490R] HistoMAX™ (GTX04404). Be the first to share your experience with this product.
![Anti-S100 beta antibody [MSVA-490R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04404](/upload/website/prouct_img/small/GTX04404/GTX04404_20230728_IHC-P_107_23072722_172.webp "Anti-S100 beta antibody [MSVA-490R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04404")
![Anti-S100 beta antibody [MSVA-490R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04404](/upload/website/prouct_img/small/GTX04404/GTX04404_20230728_IHC-P_237_23072723_372.webp "Anti-S100 beta antibody [MSVA-490R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04404")
![Anti-S100 beta antibody [MSVA-490R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04404](/upload/website/prouct_img/small/GTX04404/GTX04404_20230728_IHC-P_367_23072723_797.webp "Anti-S100 beta antibody [MSVA-490R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04404")
![Anti-S100 beta antibody [MSVA-490R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04404](/upload/website/prouct_img/normal/GTX04404/GTX04404_20230728_IHC-P_107_23072722_172.webp "Anti-S100 beta antibody [MSVA-490R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04404")
![Anti-S100 beta antibody [MSVA-490R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04404](/upload/website/prouct_img/normal/GTX04404/GTX04404_20230728_IHC-P_237_23072723_372.webp "Anti-S100 beta antibody [MSVA-490R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04404")
![Anti-S100 beta antibody [MSVA-490R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04404](/upload/website/prouct_img/normal/GTX04404/GTX04404_20230728_IHC-P_367_23072723_797.webp "Anti-S100 beta antibody [MSVA-490R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04404")