GeneTex
United States (US)

S100 beta antibody

GTX129573_41584_IHC_18121410_524.jpg
GTX129573_41584_20160204_WB_18121410_353.jpg
GTX129573_41584_WB_B_18121410_660.jpg
GTX129573_41584_20160218_WB_M_brain_18121410_622.jpg
GTX129573_41584_20170220_IHC-Fr_M_18121410_956.jpg
GTX129573_41584_20160218_WB_R_brain_18121410_164.jpg

Cat No. GTX129573

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat, Squirrel
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

V5-human S100B-transfected 293T , A375 , mouse brain , rat brain

Predict Reactivity

Rabbit, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human S100 beta Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

S100 Calcium Binding Protein B,Nef,S100,S100-B,S100Beta,S100B

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX129573_41584_IHC_18121410_524.jpg

GTX129573 IHC-P Image

S100B antibody detects S100B protein at glial cell on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
S100B antibody (GTX129573) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX129573_41584_20160204_WB_18121410_353.jpg

GTX129573 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with S100B antibody (GTX129573) diluted at 1:500.

GTX129573_41584_WB_B_18121410_660.jpg

GTX129573 WB Image

S100B antibody detects S100B protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of V5-human S100B-transfected 293T cells
15 % SDS-PAGE
S100B antibody (GTX129573) dilution: 1:1000

GTX129573_41584_20160218_WB_M_brain_18121410_622.jpg

GTX129573 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with S100B antibody (GTX129573) diluted at 1:1000.

GTX129573_41584_20170220_IHC-Fr_M_18121410_956.jpg

GTX129573 IHC-Fr Image

S100 beta antibody detects S100 beta protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: S100 beta stained by S100 beta antibody (GTX129573) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX129573_41584_20160218_WB_R_brain_18121410_164.jpg

GTX129573 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with S100B antibody (GTX129573) diluted at 1:1000.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169