IHC: Use at a dilution of 1/250. IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/500. Detects a band of approximately 12 kDa. Optimal dilutions/concentrations should be determined by the end user.
Rat brain tissue lysate
Does not cross react with other calcium binding proteins including calbindin, calretinin or parvalbumins.
PH 7.4, phosphate buffer, stabilizing proteins (includes BSA) and 0.02% Sodium Azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.6 mg/ml (Please refer to the vial label for the specific concentration.)
Full length native protein homodimer (purified) (Human).
Immunogen affinity purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
S100 Calcium Binding Protein B,Nef,S100,S100-B,S100Beta,S100B
This protein is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.