GeneTex
United States (US)

S100 beta antibody

S100-beta-antibody_WB_GTX14688-1_18121411_207.jpg

Cat No. GTX14688

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP, IHC
Reactivity Human, Mouse
APPLICATION

Application Note

IHC: Use at a dilution of 1/250. IP: Use at a dilution of 1/250. WB: Use at a dilution of 1/500. Detects a band of approximately 12 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

11 kDa. ( Note )

Positive Control

Rat brain tissue lysate

Specificity/Sensitivity

Does not cross react with other calcium binding proteins including calbindin, calretinin or parvalbumins.
PROPERTIES

Form

Liquid

Buffer

PH 7.4, phosphate buffer, stabilizing proteins (includes BSA) and 0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length native protein homodimer (purified) (Human).

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

S100 Calcium Binding Protein B,Nef,S100,S100-B,S100Beta,S100B

Background

This protein is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.

Database

Research Area

DATA IMAGES
S100-beta-antibody_WB_GTX14688-1_18121411_207.jpg

GTX14688 WB Image

Western blot of S100b with GTX14688. Antibody Dilution 1:500. 15% disconineous gel

REFERENCE
Package List Price ($)
$ 369