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S100A10 antibody

KO/KD
Anti-S100A10 antibody used in Western Blot (WB). GTX100697
Anti-S100A10 antibody used in IHC (Paraffin sections) (IHC-P). GTX100697
Anti-S100A10 antibody used in Western Blot (WB). GTX100697

Cat. No. GTX100697

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all S100A10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

A431 , 293T

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human S100A10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951799

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

S100 calcium binding protein A10 , 42C , ANX2L , ANX2LG , CAL1L , CLP11 , Ca[1] , GP11 , P11 , p10

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in exocytosis and endocytosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-S100A10 antibody used in Western Blot (WB). GTX100697

GTX100697 WB Image

KO/KD

Wild-type (WT) and S100A10 knockout (KO) 293T cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with S100A10 antibody (GTX100697) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-S100A10 antibody used in IHC (Paraffin sections) (IHC-P). GTX100697

GTX100697 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using S100A10(GTX100697) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-S100A10 antibody used in Western Blot (WB). GTX100697

GTX100697 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
15% SDS PAGE
GTX100697 diluted at 1:1000

REFERENCE

Application Reference

Zhou X et al. Front Genet 2021; 12 : 695036 S100 Calcium Binding Protein A10, A Novel Oncogene, Promotes the Proliferation, Invasion, and Migration of Hepatocellular Carcinoma.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169