GeneTex
United States (US)

S100A10 antibody

KO/KD
GTX100697_40058_20180126_WB_KO_watermark_19011506_335.jpg
GTX100697_WB_19011506_319.jpg
GTX100697_40058_IHC_19011506_613.jpg

Cat No. GTX100697

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa

Predict Reactivity

Rabbit, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human S100A10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

42C antibody, ANX2L antibody, ANX2LG antibody, CAL1L antibody, CLP11 antibody, Ca[1] antibody, GP11 antibody, MGC111133 antibody, P11 antibody, p10 antibody, S100A10 antibody, "annexin II ligand, calpactin I, light polypeptide antibody", calpactin-1 light chain antibody, "S100 calcium-binding protein A10 (annexin II ligand, calpactin I, light polypeptide (p11)) antibody", calpactin I light chain antibody, cellular ligand of annexin II antibody, "S100 calcium binding protein A10 (annexin II ligand, calpactin I, light polypeptide (p11)) antibody", protein S100-A10 antibody, S100 calcium binding protein A10 antibody

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in exocytosis and endocytosis. [provided by RefSeq]

Database

DATA IMAGES
GTX100697_40058_20180126_WB_KO_watermark_19011506_335.jpg

GTX100697 WB Image

KO/KD

Wild-type (WT) and S100A10 knockout (KO) 293T cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with S100A10 antibody (GTX100697) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CRISPR knockout cell lines generated by www.edigene.com

GTX100697_WB_19011506_319.jpg

GTX100697 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
15% SDS PAGE
GTX100697 diluted at 1:1000

GTX100697_40058_IHC_19011506_613.jpg

GTX100697 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using S100A10(GTX100697) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169