GeneTex
United States (US)

S100A11 antibody

KO/KD
GTX100698_40058_20181123_WB_KO_watermark_19011519_922.jpg
GTX100698_40058_WB_19011506_897.jpg
GTX100698_IHC_19011506_149.jpg
GTX100698_IFA_19011506_207.jpg

Cat No. GTX100698

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human S100A11. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

S100 Calcium Binding Protein A11,Hel-S-43,Mln70,S100C,S100A11

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. [provided by RefSeq]

Database

DATA IMAGES
GTX100698_40058_20181123_WB_KO_watermark_19011519_922.jpg

GTX100698 WB Image

KO/KD

Wild-type (WT) and S100A11 knockout (KO) HeLa cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with S100A11 antibody (GTX100698) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX100698_40058_WB_19011506_897.jpg

GTX100698 WB Image

S100A11 antibody detects S100A11 protein by Western blot analysis.
A. 30 µg A431 whole cell lysate/extract
15 % SDS-PAGE
S100A11 antibody (GTX100698) dilution: 1:1000

GTX100698_IHC_19011506_149.jpg

GTX100698 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using S100A11(GTX100698) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100698_IFA_19011506_207.jpg

GTX100698 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using S100A11(GTX100698) antibody at 1:200 dilution.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169