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S100A11 antibody

KO/KD
Anti-S100A11 antibody used in Western Blot (WB). GTX100698
Anti-S100A11 antibody used in IHC (Paraffin sections) (IHC-P). GTX100698
Anti-S100A11 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100698

Cat. No. GTX100698

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all S100A11 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human S100A11. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951801

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

S100 calcium binding protein A11 , HEL-S-43 , MLN70 , S100C

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-S100A11 antibody used in Western Blot (WB). GTX100698

GTX100698 WB Image

KO/KD

Wild-type (WT) and S100A11 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with S100A11 antibody (GTX100698) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-S100A11 antibody used in IHC (Paraffin sections) (IHC-P). GTX100698

GTX100698 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using S100A11(GTX100698) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-S100A11 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100698

GTX100698 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using S100A11(GTX100698) antibody at 1:200 dilution.

REFERENCE

Application Reference

Lee HY et al. Oncotarget 2018; 9 (17) : 13193-13205 EIF3C-enhanced exosome secretion promotes angiogenesis and tumorigenesis of human hepatocellular carcinoma.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169