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S100A4 antibody [S100A4/1481]

Anti-S100A4 antibody [S100A4/1481] used in Protein Array (Protein Array). GTX34999
Anti-S100A4 antibody [S100A4/1481] used in Flow cytometry (FACS). GTX34999
Anti-S100A4 antibody [S100A4/1481] used in Western Blot (WB). GTX34999
Anti-S100A4 antibody [S100A4/1481] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34999
Anti-S100A4 antibody [S100A4/1481] used in Flow cytometry (FACS). GTX34999
Anti-S100A4 antibody [S100A4/1481] used in IHC (Paraffin sections) (IHC-P). GTX34999
Anti-S100A4 antibody [S100A4/1481] used in Western Blot (WB). GTX34999

Cat. No. GTX34999

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 S100A4/1481

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, Protein Array

Reactivity

 Human, Mouse
Package
100 μg ($529)
See all S100A4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CAPL , FSP1 , MTS1 , P9KA , PEL98 , S100 calcium binding protein A4 , S100A4 , 18A2 , 42A

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-S100A4 antibody [S100A4/1481] used in Protein Array (Protein Array). GTX34999

GTX34999 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using S100A4 Mouse Monoclonal Antibody (S100A4/1481). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Anti-S100A4 antibody [S100A4/1481] used in Flow cytometry (FACS). GTX34999

GTX34999 FACS Image

FACS analysis of A549 cells using GTX34999 S100A4 antibody [S100A4/1481].
Blue : Primary antibody
Red : Isotype control

Anti-S100A4 antibody [S100A4/1481] used in Western Blot (WB). GTX34999

GTX34999 WB Image

WB analysis of HeLa cell lysate using GTX34999 S100A4 antibody [S100A4/1481].

Anti-S100A4 antibody [S100A4/1481] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34999

GTX34999 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX34999 S100A4 antibody [S100A4/1481].
Green : Primary antibody
Red : nucleus

Anti-S100A4 antibody [S100A4/1481] used in Flow cytometry (FACS). GTX34999

GTX34999 FACS Image

FACS analysis of T98G cells using GTX34999 S100A4 antibody [S100A4/1481].
Blue : Primary antibody
Red : Isotype control

Anti-S100A4 antibody [S100A4/1481] used in IHC (Paraffin sections) (IHC-P). GTX34999

GTX34999 IHC-P Image

IHC-P analysis of human placenta tissue using GTX34999 S100A4 antibody [S100A4/1481].

Anti-S100A4 antibody [S100A4/1481] used in Western Blot (WB). GTX34999

GTX34999 WB Image

WB analysis of HeLa, A549, and A375 cell lysates using GTX34999 S100A4 antibody [S100A4/1481].

REFERENCE

Application Reference

Hsin-Hsien Li et al. Part Fibre Toxicol 2021; 18 (1) : 11 Upregulation of ACE2 and TMPRSS2 by particulate matter and idiopathic pulmonary fibrosis: a potential role in severe COVID-19.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529