APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human S100A4 protein (aa1-200) (exact sequence is proprietary)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CAPL , FSP1 , MTS1 , P9KA , PEL98 , S100 calcium binding protein A4 , S100A4 , 18A2 , 42A
Background
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-S100A4 antibody [S100A4/1482] used in Flow cytometry (FACS). GTX35000](/templates/default/js/lightbox/images/loading.gif "Anti-S100A4 antibody [S100A4/1482] used in Flow cytometry (FACS). GTX35000")
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GTX35000 FACS Image
FACS analysis of A549 cells using GTX35000 S100A4 antibody [S100A4/1482].
Blue : Primary antibody
Red : Isotype control
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GTX35000 IHC-P Image
IHC-P analysis of human placenta tissue using GTX35000 S100A4 antibody [S100A4/1482].
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GTX35000 WB Image
WB analysis of HeLa, A549, and A375 cell lysates using GTX35000 S100A4 antibody [S100A4/1482].
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GTX35000 FACS Image
FACS analysis of T98G cells using GTX35000 S100A4 antibody [S100A4/1482].
Blue : Primary antibody
Red : Isotype control
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GTX35000 WB Image
WB analysis of HeLa cell lysate using GTX35000 S100A4 antibody [S100A4/1482].
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REFERENCE
There are currently no references for S100A4 antibody [S100A4/1482] (GTX35000). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for S100A4 antibody [S100A4/1482] (GTX35000). Be the first to share your experience with this product.
![Anti-S100A4 antibody [S100A4/1482] used in Flow cytometry (FACS). GTX35000](/upload/website/prouct_img/small/GTX35000/GTX35000_20200115_FACS_54_w_23060801_663.webp "Anti-S100A4 antibody [S100A4/1482] used in Flow cytometry (FACS). GTX35000")
![Anti-S100A4 antibody [S100A4/1482] used in IHC (Paraffin sections) (IHC-P). GTX35000](/upload/website/prouct_img/small/GTX35000/GTX35000_20200115_IHC-P_992_w_23060801_442.webp "Anti-S100A4 antibody [S100A4/1482] used in IHC (Paraffin sections) (IHC-P). GTX35000")
![Anti-S100A4 antibody [S100A4/1482] used in Western Blot (WB). GTX35000](/upload/website/prouct_img/small/GTX35000/GTX35000_20200115_WB_233_w_23060801_186.webp "Anti-S100A4 antibody [S100A4/1482] used in Western Blot (WB). GTX35000")
![Anti-S100A4 antibody [S100A4/1482] used in Flow cytometry (FACS). GTX35000](/upload/website/prouct_img/small/GTX35000/GTX35000_20200115_FACS_1881_w_23060801_954.webp "Anti-S100A4 antibody [S100A4/1482] used in Flow cytometry (FACS). GTX35000")
![Anti-S100A4 antibody [S100A4/1482] used in Western Blot (WB). GTX35000](/upload/website/prouct_img/small/GTX35000/GTX35000_20200115_WB_133_w_23060801_365.webp "Anti-S100A4 antibody [S100A4/1482] used in Western Blot (WB). GTX35000")
![Anti-S100A4 antibody [S100A4/1482] used in Flow cytometry (FACS). GTX35000](/upload/website/prouct_img/normal/GTX35000/GTX35000_20200115_FACS_54_w_23060801_663.webp "Anti-S100A4 antibody [S100A4/1482] used in Flow cytometry (FACS). GTX35000")
![Anti-S100A4 antibody [S100A4/1482] used in IHC (Paraffin sections) (IHC-P). GTX35000](/upload/website/prouct_img/normal/GTX35000/GTX35000_20200115_IHC-P_992_w_23060801_442.webp "Anti-S100A4 antibody [S100A4/1482] used in IHC (Paraffin sections) (IHC-P). GTX35000")
![Anti-S100A4 antibody [S100A4/1482] used in Western Blot (WB). GTX35000](/upload/website/prouct_img/normal/GTX35000/GTX35000_20200115_WB_233_w_23060801_186.webp "Anti-S100A4 antibody [S100A4/1482] used in Western Blot (WB). GTX35000")
![Anti-S100A4 antibody [S100A4/1482] used in Flow cytometry (FACS). GTX35000](/upload/website/prouct_img/normal/GTX35000/GTX35000_20200115_FACS_1881_w_23060801_954.webp "Anti-S100A4 antibody [S100A4/1482] used in Flow cytometry (FACS). GTX35000")
![Anti-S100A4 antibody [S100A4/1482] used in Western Blot (WB). GTX35000](/upload/website/prouct_img/normal/GTX35000/GTX35000_20200115_WB_133_w_23060801_365.webp "Anti-S100A4 antibody [S100A4/1482] used in Western Blot (WB). GTX35000")