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S100A4 antibody [S100A4/1482]

Anti-S100A4 antibody [S100A4/1482] used in IHC (Paraffin sections) (IHC-P). GTX35000
Anti-S100A4 antibody [S100A4/1482] used in Western Blot (WB). GTX35000

Cat No. GTX35000

Host Mouse
Clonality Monoclonal
Clone Name S100A4/1482
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Specificity/Sensitivity

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, α6β4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human S100A4 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

S100 Calcium Binding Protein A4,18A2,42A,Capl,Fsp1,Mts1,P9Ka,Pel98,S100A4

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-S100A4 antibody [S100A4/1482] used in IHC (Paraffin sections) (IHC-P). GTX35000

GTX35000 IHC-P Image

Formalin--paraffin human Placenta stained with S100A4 Monoclonal Antibody (S100A4/1482).

Anti-S100A4 antibody [S100A4/1482] used in Western Blot (WB). GTX35000

GTX35000 WB Image

Western Blot of HeLa, A549 and A375 Cell Lysate using S100A4 Monoclonal Antibody (S100A4/1482).

Package List Price ($)
$ 329