S100A4 antibody [S100A4/2750R]

Cat. No. GTX02703

Host	Rabbit
Clonality	Monoclonal
Clone Name	S100A4/2750R
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human, Mouse
Package	100 μg ($529)

See all S100A4 products

Datasheet

View product citations for antibody GTX02703 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2 μg/ml
ICC/IF	1-2 μg/ml
IHC-P	1-2 μg/ml
FACS	1-2 μg/million cells
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Observed MW

10-12 kDa.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human S100A4 protein (around aa1-200)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

S100 calcium binding protein A4 , 18A2 , 42A , CAPL , FSP1 , MTS1 , P9KA , PEL98

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Database

Gene ID: 6275 S100A4
UniProt: P26447 S100A4

Research Area

DATA IMAGES

GTX02703 IHC-P Image

IHC-P analysis of human placenta tissue section using GTX02703 S100A4 antibody [S100A4/2750R].

GTX02703 WB Image

WB analysis of HeLa whole cell lysate using GTX02703 S100A4 antibody [S100A4/2750R].

GTX02703 FACS Image

FACS analysis of A549 cells using GTX02703 S100A4 antibody [S100A4/2750R].
Blue : Primary antibody
Red : Isotype control

GTX02703 ICC/IF Image

ICC/IF analysis of A549 cells using GTX02703 S100A4 antibody [S100A4/2750R].
Green : Primary antibody
Red : nuclear counterstain

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 529

Qty