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S100A4 antibody

KO/KD
Anti-S100A4 antibody used in IHC (Paraffin sections) (IHC-P). GTX32855
Anti-S100A4 antibody used in IHC (Paraffin sections) (IHC-P). GTX32855
Anti-S100A4 antibody used in Western Blot (WB). GTX32855
Anti-S100A4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32855
Anti-S100A4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32855
Anti-S100A4 antibody used in Western Blot (WB). GTX32855
Anti-S100A4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32855

Cat. No. GTX32855

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all S100A4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human S100A4 (NP_002952.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887683

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

S100 calcium binding protein A4 , 18A2 , 42A , CAPL , FSP1 , MTS1 , P9KA , PEL98

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-S100A4 antibody used in IHC (Paraffin sections) (IHC-P). GTX32855

GTX32855 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX32855 S100A4 antibody.
Dilution : 1:100

Anti-S100A4 antibody used in IHC (Paraffin sections) (IHC-P). GTX32855

GTX32855 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX32855 S100A4 antibody.
Dilution : 1:100

Anti-S100A4 antibody used in Western Blot (WB). GTX32855

GTX32855 WB Image

WB analysis of various sample lysates using GTX32855 S100A4 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-S100A4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32855

GTX32855 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32855 S100A4 antibody.
Blue : DAPI
Dilution : 1:100

Anti-S100A4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32855

GTX32855 ICC/IF Image

ICC/IF analysis of Raw264.7 cells using GTX32855 S100A4 antibody.
Blue : DAPI
Dilution : 1:100

Anti-S100A4 antibody used in Western Blot (WB). GTX32855

GTX32855 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX32855 S100A4 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-S100A4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32855

GTX32855 ICC/IF Image

ICC/IF analysis of PC-12 cells using GTX32855 S100A4 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

Application Reference

Zhao B et al. J Proteomics 2015; 112 : 38-52 Comprehensive proteome quantification reveals NgBR as a new regulator for epithelial-mesenchymal transition of breast tumor cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399