APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Note :
WB
non-reduced;~22 kDa; Doesn’t recognize the single proteins S100A8 and S100A9
IHC-Fr
acetone fixation; 0.1 % hydrogen peroxide treatment to reduce enogenous peroxidise activity; positive control: inflammatory tissue; negative control: normal human tissue (skin, lung, colon).
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Not tested in other applications.
Calculated MW
Product Note
The monoclonal antibody 27E10 recognizes an epitope specific for the S100A8/A9 heterocomplex that is not exposed on the individual subunits S100A8 (MRP8, calgranulin-A) or S100A9 (MRP14, calgranulin-B). The monoclonal antibody 27E10 can be used for early detection of inflammatory macrophages, for the characterization of tumorous tissues and the monitoring of peripheral blood cell cultures. The antibody 27E10 does not react with lymphocytes or platelets.
We do not recommend use of this product for Mouse samples.
PROPERTIES
Form
Liquid
Buffer
0.2μm-filtered PBS, 0.1% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
100 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Human blood monocytes
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CAGA,CFAG,CGLA,CP10,L1Ag,MA387,MIF,MRP8,NIF,P8,S100 calcium binding protein A8,S100A8,Calgranulin A,60B8AG
Cellular Localization
Secreted,Cytoplasm,Cytoskeleton,Cell membrane
Background
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]
Database
Research Area
DATA IMAGES
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GTX17050 ELISA Image
ELISA analysis of human blood monocytes using GTX17050 S100A8 + S100A9 antibody [27E10]. Dilution : 0.5-2 μg/ml
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GTX17050 IHC-Fr Image
IHC-Fr analysis of human squamous epithelia of tonsil section using GTX17050 S100A8 + S100A9 antibody [27E10].
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GTX17050 FACS Image
FACS analysis of human peripheral blood mononuclear cells (PBMC) using GTX17050 S100A8 + S100A9 antibody [27E10]. Light Green and Black : Isotype control in 2 concentrations Pink : Secondary antibody only Dark Green and Brown line : in a concentration of 1 and 3 μl/2x10⁵ cells
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REFERENCE
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REVIEW
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