S100A8 + S100A9 antibody [27E10]

Cat. No. GTX17050

Host	Mouse
Clonality	Monoclonal
Clone Name	27E10
Isotype	IgG1
Application	WB, ICC/IF, IHC-Fr, FACS, IP, ELISA
Reactivity	Human, Monkey
Package	50 μg ($399)

See all S100A8 + S100A9 products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-Fr	Assay dependent
FACS	Assay dependent
IP	Assay dependent
ELISA	Assay dependent
Note : WB non-reduced;~22 kDa; Doesn’t recognize the single proteins S100A8 and S100A9 IHC-Fr acetone fixation; 0.1 % hydrogen peroxide treatment to reduce enogenous peroxidise activity; positive control: inflammatory tissue; negative control: normal human tissue (skin, lung, colon).

Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Product Note

The monoclonal antibody 27E10 recognizes an epitope specific for the S100A8/A9 heterocomplex that is not exposed on the individual subunits S100A8 (MRP8, calgranulin-A) or S100A9 (MRP14, calgranulin-B). The monoclonal antibody 27E10 can be used for early detection of inflammatory macrophages, for the characterization of tumorous tissues and the monitoring of peripheral blood cell cultures. The antibody 27E10 does not react with lymphocytes or platelets.
We do not recommend use of this product for Mouse samples.

PROPERTIES

Form

Liquid

Buffer

0.2μm-filtered PBS, 0.1% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human blood monocytes

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CAGA,CFAG,CGLA,CP10,L1Ag,MA387,MIF,MRP8,NIF,P8,S100 calcium binding protein A8,S100A8,Calgranulin A,60B8AG

Cellular Localization

Secreted,Cytoplasm,Cytoskeleton,Cell membrane

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]

Database

Gene ID: 6279 S100A8
Gene ID: 6280
UniProt: P05109 S100A8

Research Area

DATA IMAGES

GTX17050 ELISA Image

ELISA analysis of human blood monocytes using GTX17050 S100A8 + S100A9 antibody [27E10].
Dilution : 0.5-2 μg/ml

GTX17050 IHC-Fr Image

IHC-Fr analysis of human squamous epithelia of tonsil section using GTX17050 S100A8 + S100A9 antibody [27E10].

GTX17050 FACS Image

FACS analysis of human peripheral blood mononuclear cells (PBMC) using GTX17050 S100A8 + S100A9 antibody [27E10].
Light Green and Black : Isotype control in 2 concentrations
Pink : Secondary antibody only
Dark Green and Brown line : in a concentration of 1 and 3 μl/2x10⁵ cells

REFERENCE

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REVIEW

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Package

List Price ($)

50 μg

$ 399

Qty