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S100A8 antibody

Anti-S100A8 antibody used in IHC (Paraffin sections) (IHC-P). GTX02317
Anti-S100A8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02317
Anti-S100A8 antibody used in Western Blot (WB). GTX02317

Cat No. GTX02317

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:300-1:10000
IHC-P 1:5000-1:100000
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.2μm-filtered PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Human S100A8 / MRP-8 protein

Purification

Purified by Protein A and Antigen Affinity

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

S100 calcium binding protein A8 , 60B8AG , CAGA , CFAG , CGLA , CP-10 , L1Ag , MA387 , MIF , MRP8 , NIF , P8

Cellular Localization

Secreted , Cytoplasm , Cytoplasm, cytoskeleton

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-S100A8 antibody used in IHC (Paraffin sections) (IHC-P). GTX02317

GTX02317 IHC-P Image

IHC-P analysis of human spleen tissue section using GTX02317 S100A8 antibody.
Dilution : 1:10000

Anti-S100A8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02317

GTX02317 ICC/IF Image

ICC/IF analysis of PFA-fixed MCF-7 cells using GTX02317 S100A8 antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 1:1000
Permeabilzation : 0.1% Triton X-100

Anti-S100A8 antibody used in Western Blot (WB). GTX02317

GTX02317 WB Image

WB analysis of HL-60 whole cell lysate (Lane A) using GTX02317 S100A8 antibody.
Dilution : 1:500
Loading : 30 μg

REFERENCE

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REVIEW

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Package List Price ($)
$ 339