GeneTex
United States (US)

S100A9 antibody, Internal

GTX88481_2288_WB_18122102_970.jpg
GTX88481_947_IHC-P_18122102_791.jpg
GTX88481_948_IHC-P_18122102_975.jpg

Cat No. GTX88481

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
IHC-P 2.5μg/ml

Note :

IHC-P
Human Lung shows nuclear staining in select cells.

Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88481-PEP).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DTNADKQLSFEEF , from the internal region of the protein sequence according to NP_002956.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

S100 Calcium Binding Protein A9,60B8Ag,Cagb,Cfag,Cglb,L1Ag,Liag,Mac387,Mif,Mrp14,Nif,P14,S100A9

Cellular Localization

Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88481_2288_WB_18122102_970.jpg

GTX88481 WB Image

WB analysis of human peripheral mononucleocytes lysate using GTX88481 S100A9 antibody, Internal.
Dilution : 0.5µg/ml
Loading : 35µg protein in RIPA buffer

GTX88481_947_IHC-P_18122102_791.jpg

GTX88481 IHC-P Image

IHC-P analysis of human liver using GTX88481 S100A9 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5µg/ml

GTX88481_948_IHC-P_18122102_975.jpg

GTX88481 IHC-P Image

IHC-P analysis of human lung using GTX88481 S100A9 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5µg/ml

Package List Price ($)
$ 329