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S100A9 antibody [S100A9/1011]

Anti-S100A9 antibody [S100A9/1011] used in IHC (Paraffin sections) (IHC-P). GTX35018

Cat No. GTX35018

Host Mouse
Clonality Monoclonal
Clone Name S100A9/1011
Isotype IgM
Application ICC/IF, IHC-P, IHC-Fr
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT
IHC-Fr 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Specificity/Sensitivity

Recognizes a 14kDa protein, identified as S100A9 (also known as Calgranulin B or MRP-14); expressed by granulocytes, monocytes and by tissue macrophages.ĀThe protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This MAb reacts with neutrophils, monocytes and macrophages, and has been shown as an important marker for identifying macrophages in tissue sections. Among cells that are now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human S100A9 protein

Purification

Ab purified from Bioreactor Concentrate

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

S100 Calcium Binding Protein A9,60B8Ag,Cagb,Cfag,Cglb,L1Ag,Liag,Mac387,Mif,Mrp14,Nif,P14,S100A9

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-S100A9 antibody [S100A9/1011] used in IHC (Paraffin sections) (IHC-P). GTX35018

GTX35018 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with S100A9 Monoclonal Antibody (S100A9/1011)

Package List Price ($)
$ 329